GTF2F1 Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 10093-2-AP

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Freight/Packing

Con: 42 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
K-562 cells

Positive IP detected in:
K-562 cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
general transcription factor IIF, polypeptide 1, 74kDa

Calculated molecular weight:
58 kDa

Observed molecular weight:
74 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
GTF2F1

Synonyms
BTF4, GTF2F1, RAP74, TF2F1, TFIIF, TFIIF alpha
Background

In eukaryotic systems, the initiation of gene transcription involves the ordered assembly of a multiprotein complex on proximal promoter elements, consisting of RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, B, D, E, F and H; and sequence specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. TFIIF, a heteromer composed of a small (RAP 30) and a large (RAP 74) subunit, acting at an intermediate stage in initiation complex formation, binds directly to RNA polymerase II in solution and decrease the affinity of RNA polymerase II for nonspecific DNA. In addition, TFIIF stimulates transcription elongation by RNA polymerase II.


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