GTF2H2 Polyclonal antibody

GTF2H2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16005-1-AP

Synonyms

BTF2, BTF2 p44, BTF2P44, GTF2H2, T BTF2P44, TFIIH



Tested Applications

Positive WB detected inK-562 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

16005-1-AP targets GTF2H2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GTF2H2 fusion protein Ag8653
Full Name general transcription factor IIH, polypeptide 2, 44kDa
Calculated molecular weight395aa,44 kDa; 165aa,19 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC005345
Gene symbol GTF2H2
Gene ID (NCBI) 2966
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transcription factor IIH(TFIIH) is associated with the RNA polymerase II transcription complex, which is involved in transcription and transcription-mediated DNA repair. GTF2H2(GENERAL TRANSCRIPTION FACTOR IIH, POLYPEPTIDE 2) is one of the six subnuits forming the core-TFIIH basal transcriptional factor. TFIIH plays a role in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. The N terminus of GTF2H2 interacts with and regulates XPD, and an intact C terminus is required for a successful escape of RNAPol II from the promoter. This is a rabbit polyclonal antibody producted by part of N-terminal GTF2H2 of human origin.

Protocols

Product Specific Protocols
WB protocol for GTF2H2 antibody 16005-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

CLP1 Founder Mutation Links tRNA Splicing and Maturation to Cerebellar Development and Neurodegeneration.

Authors - Ashleigh E Schaffer