|Positive WB detected in||K-562 cells, HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Sample-dependent, check data in validation data gallery|
16005-1-AP targets GTF2H2 in WB, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||GTF2H2 fusion protein Ag8653|
|Full Name||general transcription factor IIH, polypeptide 2, 44kDa|
|Calculated molecular weight||395aa,44 kDa; 165aa,19 kDa|
|Observed molecular weight||44 kDa|
|GenBank accession number||BC005345|
|Gene ID (NCBI)||2966|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Transcription factor IIH(TFIIH) is associated with the RNA polymerase II transcription complex, which is involved in transcription and transcription-mediated DNA repair. GTF2H2(GENERAL TRANSCRIPTION FACTOR IIH, POLYPEPTIDE 2) is one of the six subnuits forming the core-TFIIH basal transcriptional factor. TFIIH plays a role in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. The N terminus of GTF2H2 interacts with and regulates XPD, and an intact C terminus is required for a successful escape of RNAPol II from the promoter. This is a rabbit polyclonal antibody producted by part of N-terminal GTF2H2 of human origin.