Galc Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 11991-1-AP

Featured Product KD/KO validated

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Con: 51 μg/150 μl

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Species specificity:
human, mouse, rat, zebrafish

Positive WB detected in:
mouse brain tissue, A375 cells

Positive IP detected in:
NIH/3T3 cells

Positive IHC detected in:
human gliomas tissue

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
galactosylceramidase

Calculated molecular weight:
77 kDa

Observed molecular weight:
80 kDa,30 kDa, 50 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
Galc

Synonyms
2310068B06Rik, A930008M05Rik, AW212969, AW413532, Gacy, galactosylceramidase, Galc, twi, twitcher
Background

The GALC antibody targets the liposomal enzyme Galactosylceramidase (GALC), which belongs to the glycosyl hydrolase 59 family. It hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. It is primarily found in the brain and kidneys where galactolipids are hydrolyzed(PMID:8634707). Deficiencies of GALC are primarily associated with the autosomal recessive Krabbe’s disease. This disease is characterized by developmental delay caused by apoptosis of myelin-forming cells. GALC is responsible for hydrolyzing galactosylceramide, a cerebroside that is an important component of myelin. A deficiency in GALC causes loss of myelin to nerve cells, resulting in delayed nerve transmissions. Krabbe’s disease has varying degrees of severity due to a large number of different genetic mutations in the gene. The GALC antibody can be used to detect the deletions in the GALC gene and functions of the enzyme(PMID:20886637).Normal GALC mRNA encodes the 80 kDa precursor, which is processed into 50 and 30 kDa subunits. (PMID:8634707 )


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