Glypican 3 Polyclonal antibody

Glypican 3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 25175-1-AP

Synonyms

DGSX, Glypican 3, GPC3, GTR2 2, Intestinal protein OCI 5, MXR7, OCI 5, OCI5, SDYS, SGB, SGBS, SGBS1



Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, HEK-293 cell line

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25175-1-AP is Glypican 3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Glypican 3 fusion protein Ag10129
Full Name glypican 3
Calculated Molecular Weight 580 aa, 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC035972
Gene Symbol GPC3
Gene ID (NCBI) 2719
RRIDAB_2879942
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glypicans (GPCs) are a family of glycosylphosphatidylinositol (GPI)-anchored heparan sulphate proteoglycans (HSPGs) that may play a role in the control of cell division and growth regulation. In mammals, there are six GPCs (GPC1 to GPC6), all of which have a similar core-protein size of approx. 60 kDa and the clustering of glycosaminoglycan attachment site near the C-terminus. They are tethered to the cell surface by GPI linkages, which can be cleaved by endogenous phospholipases, thus releasing the protein. Glypican 3 (GPC3) is highly expressed in many tissues during development and plays an important role in the regulation of embryonic growth (PMID: 22467855). Loss-of-function mutations of GPC3 result in the Simpson-Golabi-Behmel overgrowth syndrome (SGBS), and Gpc-3 null mice display developmental overgrowth (PMID: 8589713; 18477453). In hepatocellular carcinoma (HCC), the overexpression of glypican 3 has been demonstrated to be a reliable diagnostic indicator (PMID: 19212669; 22706665). The calculated molecular weight of native glypican 3 is 66 kDa, glycanated forms of glypican 3 have higher molecular weights than 66 kDa (PMID: 12851874; 16024626; 19574424).

Protocols

Product Specific Protocols
WB protocol for Glypican 3 antibody 25175-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Hepatology

The Mutational Features of Aristolochic Acid-Induced Mouse and Human Liver Cancers.

Authors - Zhao-Ning Lu
humanIF

Acta Biomater

Enhanced glypican-3-targeted identification of hepatocellular carcinoma with liver fibrosis by pre-degrading excess fibrotic collagen

Authors - Jiaming Zhuo
human

Cancer Res

hPCL3s promotes hepatocellular carcinoma metastasis by activating β-catenin signaling.

Authors - Zhen Cai
mouseWB

Redox Biol

Butaselen prevents hepatocarcinogenesis and progression through inhibiting thioredoxin reductase activity.

Authors - Xiaoqing Zheng
mouseWB

Cancers (Basel)

Therapeutic Adenovirus Vaccine Combined Immunization with IL-12 Induces Potent CD8+ T Cell Anti-Tumor Immunity in Hepatocellular Carcinoma

Authors - Samuel C Mok
humanWB

Front Oncol

TCF11 Has a Potent Tumor-Repressing Effect Than Its Prototypic Nrf1α by Definition of Both Similar Yet Different Regulatory Profiles, With a Striking Disparity From Nrf2.

Authors - Meng Wang