Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, HEK-293 cell line |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25175-1-AP targets Glypican 3 in WB, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10129 Product name: Recombinant human GPC3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 232-580 aa of BC035972 Sequence: ALNLGIEVINTTDHLKFSKDCGRMLTRMWYCSYCQGLMMVKPCGGYCNVVMQGCMAGVVEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNAGKLTTTIGKLCAHSQQRQYRFAYYPEDLFIDKKVLKVAHVEHEETLSSRRRELIQKLKSFISFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSMPKGRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMIKVKNQLRFLAELAYDLDVDDAPGNSQQATPKDNEISTFHNLGNVHSPLKLLTSMAISVVCFFFLVH Predict reactive species |
| Full Name | glypican 3 |
| Calculated Molecular Weight | 580 aa, 66 kDa |
| Observed Molecular Weight | 66 kDa |
| GenBank Accession Number | BC035972 |
| Gene Symbol | GPC3 |
| Gene ID (NCBI) | 2719 |
| RRID | AB_2879942 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51654 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glypicans (GPCs) are a family of glycosylphosphatidylinositol (GPI)-anchored heparan sulphate proteoglycans (HSPGs) that may play a role in the control of cell division and growth regulation. In mammals, there are six GPCs (GPC1 to GPC6), all of which have a similar core-protein size of approx. 60 kDa and the clustering of glycosaminoglycan attachment site near the C-terminus. They are tethered to the cell surface by GPI linkages, which can be cleaved by endogenous phospholipases, thus releasing the protein. Glypican 3 (GPC3) is highly expressed in many tissues during development and plays an important role in the regulation of embryonic growth (PMID: 22467855). Loss-of-function mutations of GPC3 result in the Simpson-Golabi-Behmel overgrowth syndrome (SGBS), and Gpc-3 null mice display developmental overgrowth (PMID: 8589713; 18477453). In hepatocellular carcinoma (HCC), the overexpression of glypican 3 has been demonstrated to be a reliable diagnostic indicator (PMID: 19212669; 22706665). The calculated molecular weight of native glypican 3 is 66 kDa, glycanated forms of glypican 3 have higher molecular weights than 66 kDa (PMID: 12851874; 16024626; 19574424).
Publications
What published studies show
Glypican 3 Polyclonal antibody (Cat# 25175-1-AP) has 11 total citations published in journals including Cancer Research, Hepatology, Redox Biology, Acta Pharmaceutica Sinica B, Acta Biomaterialia, Life Sciences, Cancers, iScience, Diagnostics, Frontiers in Oncology, and Frontiers in Pediatrics. Associated research fields include hepatocellular carcinoma, liver cancer metastasis, tumor immunotherapy, proteomics, and biliary atresia.
| Species | Application | Title |
|---|---|---|
Cancer Res hPCL3s promotes hepatocellular carcinoma metastasis by activating β-catenin signaling. | ||
Hepatology The Mutational Features of Aristolochic Acid-Induced Mouse and Human Liver Cancers. | ||
Redox Biol Butaselen prevents hepatocarcinogenesis and progression through inhibiting thioredoxin reductase activity. | ||
Acta Pharm Sin B GPC3-mediated lysosome-targeting chimeras (GLTACs) for targeted degradation of membrane proteins | ||
Acta Biomater Enhanced glypican-3-targeted identification of hepatocellular carcinoma with liver fibrosis by pre-degrading excess fibrotic collagen | ||
Life Sci The effect of hormones insulin and glucagon on ubiquitin modifications elucidated by proteomics in liver cells |
