HELZ Polyclonal antibody
HELZ Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 26635-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 26635-1-AP is HELZ Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HELZ fusion protein Ag24326 |
Full Name | helicase with zinc finger |
Calculated Molecular Weight | 1942 aa, 219 kDa |
Observed Molecular Weight | 200 kDa |
GenBank Accession Number | BC130582 |
Gene Symbol | HELZ |
Gene ID (NCBI) | 9931 |
RRID | AB_2880584 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for HELZ antibody 26635-1-AP | Download protocol |
IHC protocol for HELZ antibody 26635-1-AP | Download protocol |
IF protocol for HELZ antibody 26635-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ramona (Verified Customer) (11-12-2019) | It worked well for WB as I know which band to look at based on molecular weight. It stains a lot of non-specific molecules in IF, which seem to include something like actin fibers
|
FH Ramona (Verified Customer) (09-04-2019) | Our own custom antibody: I used an antibody customary made by a commercial company. The best crude serum of our choice was affinity purified.Problems: los of non-specific bands, cannot re-use antibody solution for the next membrane. Sometimes does not work and do not show any band even in control.Proteintech antibody: strong band of specific protein. Knock down shows expression but reduced compared to controls, which is more realistic than zero expression in all the cells.
|