HELZ Polyclonal antibody

HELZ Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 26635-1-AP

Synonyms

DHRC, DRHC, helicase with zinc finger, HELZ, HUMORF5, KIAA0054



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26635-1-AP is HELZ Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HELZ fusion protein Ag24326
Full Name helicase with zinc finger
Calculated Molecular Weight 1942 aa, 219 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberBC130582
Gene Symbol HELZ
Gene ID (NCBI) 9931
RRIDAB_2880584
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HELZ antibody 26635-1-APDownload protocol
IHC protocol for HELZ antibody 26635-1-APDownload protocol
IF protocol for HELZ antibody 26635-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ramona (Verified Customer) (11-12-2019)

It worked well for WB as I know which band to look at based on molecular weight. It stains a lot of non-specific molecules in IF, which seem to include something like actin fibers

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: IF 1:1000, WB 1:600
  • Cell Tissue Type: Human U2OS cell line
HELZ Antibody Immunofluorescence, validation (IF 1:1000, WB 1:600 dilution) in Human U2OS cell line (Cat no:26635-1-AP)
FH

Ramona (Verified Customer) (09-04-2019)

Our own custom antibody: I used an antibody customary made by a commercial company. The best crude serum of our choice was affinity purified.Problems: los of non-specific bands, cannot re-use antibody solution for the next membrane. Sometimes does not work and do not show any band even in control.Proteintech antibody: strong band of specific protein. Knock down shows expression but reduced compared to controls, which is more realistic than zero expression in all the cells.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1/600
  • Cell Tissue Type: U2OS cell
HELZ Antibody Western Blot,Immunofluorescence, validation (1/600 dilution) in U2OS cell (Cat no:26635-1-AP)