HEY1 Antibody

HEY1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, chIP, ELISA

Conjugate

Unconjugated

Cat no : 19929-1-AP

Synonyms

BHLHb31, CHF 2, CHF2, HERP2, HESR 1, HESR1, HEY1, hHRT1, HRT 1, HRT1, OAF1



Tested Applications

Positive WB detected inmouse testis tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 19929-1-AP immunogen is Fusion Protein HEY1 protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HEY1 fusion protein Ag13906
Full Name hairy/enhancer-of-split related with YRPW motif 1
Calculated molecular weight 304aa,33 kDa
Observed molecular weight 32-34 kDa
GenBank accession number BC001873
Gene symbol HEY1
Gene ID (NCBI) 23462
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hairy/enhancer of split-related proteins are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. And HEY1 is one of such protein. It is the direct downstream effector of Notch signaling which may be required for cardiovascular development. It preferentially bind to the canonical E box sequence 5'-CACGTG-3' and acts as a transcriptional repressor. HEY1 is a 33kDa protein and forms dimer by self-associating.

Protocols

Product Specific Protocols
WB protocol for HEY1 antibody 19929-1-APDownload protocol
IHC protocol for HEY1 antibody 19929-1-APDownload protocol
IP protocol for HEY1 antibody 19929-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Cell Biol

MicroRNA-34a attenuates VEGF-mediated retinal angiogenesis via targeting the Notch1.

Authors - Shaoyang Shi
mousechIP

FASEB J

Undifferentiated spermatogonia regulate Cyp26b1 expression through NOTCH signaling and drive germ cell differentiation.

Authors - Parag A Parekh
humanWB

Hepatology

A Noncoding Regulatory RNAs Network Driven by Circ-CDYL Acts Specifically in the Early Stages Hepatocellular Carcinoma.

Authors - Yanping Wei

Mol Med Rep

Salidroside suppresses the metastasis of hepatocellular carcinoma cells by inhibiting the activation of the Notch1 signaling pathway.

Authors - Linlin Lu
humanWB

Oncotarget

miR-150 enhances apoptotic and anti-tumor effects of paclitaxel in paclitaxel-resistant ovarian cancer cells by targeting Notch3.

Authors - Tae Hoen Kim
humanWB

J Hypertens

2B.06: EPIGALLOCATECHIN-3-GALLATE ATTENUATES URIC ACID-INDUCED INFLAMMATORY RESPONSES AND OXIDATIVE STRESS BY MODULATING NOTCH PATHWAY.

Authors - H Xie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Zhenminm (Verified Customer) (07-10-2019)

It is too much non-specific background.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: Mouse Testis
HEY1 Antibody Western Blot, validation (1:1,000 dilution) in Mouse Testis (Cat no:19929-1-AP)