HIF1a Antibody 52 Publications

Rabbit Polyclonal| Catalog number: 20960-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 48 μg/150 μl

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Species specificity:
human

Positive WB detected in:
Cobalt Chloride treated HeLa cells, mouse small intestine tissue

Positive IHC detected in:
human thyroid cancer tissue, human heart tissue, human kidney tissue

Positive IF detected in:
Hela cells

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:200-1:1000
IHC : 1:50-1:500
IF : 1:10-1:100

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)

Calculated molecular weight:
826aa,93 kDa

Observed molecular weight:
120 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
HIF1A

Synonyms
ARNT interacting protein, bHLHe78, HIF 1 alpha, HIF 1alpha, HIF1, HIF1 ALPHA, HIF1A, HIF-1α, Member of PAS protein 1, MOP1, PASD8
Background

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIFA), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .


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