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HIF1a Antibody 93 Publications

Rabbit Polyclonal| Catalog number: 20960-1-AP

Featured Product KD/KO validated

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Con: 48 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, CoIP, ELISA
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Images See all

HIF1a Antibody IF Cobalt Chloride treated HeLa cells 20960-1-AP

Immunofluorescent analysis of ( -20°C Acetone) fixed Cobalt Chloride treated HeLa cells (left) and untreaed cell (right) using 20960-1-AP (HIF1a antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

HIF1a Antibody WB Cobalt Chloride treated HeLa cells 20960-1-AP

Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 20960-1-AP( HIF1a Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

HIF1a Antibody IHC human thyroid cancer tissue 20960-1-AP

Immunohistochemical of paraffin-embedded human thyroid cancer using 20960-1-AP(HIF1A antibody) at dilution of 1:50 (under 40x lens)

HIF1a Antibody IP HeLa cells 20960-1-AP

IP Result of anti-HIF1a (IP:20960-1-AP, 4ug; Detection:20960-1-AP 1:300) with HeLa cells lysate 4000ug.

HIF1a Antibody FC HeLa cells 20960-1-AP

1X10^6 HeLa cells were stained with 0.2ug HIF1a antibody (20960-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human
Positive WB detected in:
Cobalt Chloride treated HeLa cells, HeLa cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human thyroid cancer tissue, human heart tissue, human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
Cobalt Chloride treated HeLa cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
HIF1a fusion protein Ag15198
Full name:
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Calculated molecular weight:
826aa,93 kDa
Observed molecular weight:
120 kDa
GenBank accession number:
BC012527
Gene ID (NCBI):
3091
Gene symbol
HIF1A
Synonyms
ARNT interacting protein, bHLHe78, HIF 1 alpha, HIF 1alpha, HIF1, HIF1 ALPHA, HIF1A, HIF-1α, Member of PAS protein 1, MOP1, PASD8

Background

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .


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