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- KD/KO Validated
HIF1a Polyclonal antibody
HIF1a Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IHC, IF, FC, CoIP, ChIP,ELISA
Conjugate
Unconjugated
220
Cat no : 20960-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Cobalt Chloride treated HeLa cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human thyroid cancer tissue, mouse kidney tissue, human heart tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Cobalt Chloride treated HeLa cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 23 publications below |
| WB | See 181 publications below |
| IHC | See 44 publications below |
| IF | See 21 publications below |
| IP | See 3 publications below |
| CoIP | See 1 publications below |
| ChIP | See 3 publications below |
Product Information
The immunogen of 20960-1-AP is HIF1a Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | chicken, human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HIF1a fusion protein Ag15198 |
| Full Name | hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) |
| Calculated molecular weight | 826 aa, 93 kDa |
| Observed molecular weight | 120 kDa |
| GenBank accession number | BC012527 |
| Gene symbol | HIF1A |
| Gene ID (NCBI) | 3091 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HIF1a antibody 20960-1-AP | Download protocol |
| IHC protocol for HIF1a antibody 20960-1-AP | Download protocol |
| IF protocol for HIF1a antibody 20960-1-AP | Download protocol |
| IP protocol for HIF1a antibody 20960-1-AP | Download protocol |
| FC protocol for HIF1a antibody 20960-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Coordinative metabolism of glutamine carbon and nitrogen in proliferating cancer cells under hypoxia. | ||
J Physiol Chronic fetal hypoxia disrupts the peri-conceptual environment in next-generation adult female rats. | ||
Front Oncol Targeting JUN, CEBPB, and HDAC3: A Novel Strategy to Overcome Drug Resistance in Hypoxic Glioblastoma. | ||
Theranostics Oncogenic MSH6-CXCR4-TGFB1 Feedback Loop: A Novel Therapeutic Target of Photothermal Therapy in Glioblastoma Multiforme. | ||
Cancer Lett UHRF1 promotes aerobic glycolysis and proliferation via suppression of SIRT4 in pancreatic cancer. | ||
Onco Targets Ther Hypoxic non-small-cell lung cancer cell-derived exosomal miR-21 promotes resistance of normoxic cell to cisplatin. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Diane (Verified Customer) (02-17-2021) | Separated on a 7.5% SDS PAGE gel, transferred to nitrocellulose membrane. Block with 5% dry milk/PBST at room temperature for one hour. 1:1000 dilution of the primary antibody in blocking solution. Incubated overnight at 4 degrees C on a rocker platform. Secondary antibody 1:1000 secondary (anti rabbit HRP) incubation for two hours at room temperature. Visualization with the Opti-4CN chromogenic substrate kit (BioRad).
 |
FH Chun (Verified Customer) (12-05-2019) | A good antibody
|
