Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 20960-1-AP( HIF1a Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 20960-1-AP( HIF1a Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

Whole cell lysate from HeLa cells that were either treated with cobalt chloride (+; 500 uM) or mock treated (-) and were subjected to SDS PAGE followed by western blot with 20960-1-AP( HIF1a Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 20960-1-AP( HIF1a Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

Immunohistochemical of paraffin-embedded human heart using 20960-1-AP(HIF1A antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human heart using 20960-1-AP(HIF1A antibody) at dilution of 1:100 (under 40x lens)

Immunohistochemical of paraffin-embedded human kidney using 20960-1-AP(HIF1A antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human kidney using 20960-1-AP(HIF1A antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemical of paraffin-embedded human thyroid cancer using 20960-1-AP(HIF1A antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human thyroid cancer using 20960-1-AP(HIF1A antibody) at dilution of 1:50 (under 40x lens)

Immunofluorescent analysis of Hela cells, using HIF1A antibody 20960-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

1X10^6 HeLa cells were stained with 0.2ug HIF1a antibody (20960-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.