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KD/KO Validated

HIF1a Polyclonal antibody

Name: HIF1a Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 20960-1-AP
Rating: 3.00 (1 reviews)

HIF1a Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, chIP,ELISA

Conjugate

Unconjugated

Publications

195

Reviews (1)

Cat no : 20960-1-AP

Synonyms

ARNT interacting protein, bHLHe78, HIF 1 alpha, HIF 1alpha, HIF1, HIF1 ALPHA, HIF1A, HIF-1α, Member of PAS protein 1, MOP1, PASD8

Validation Data Gallery

IF Staining of HeLa using 20960-1-AP
Immunofluorescent analysis of (-20°C Acetone) fixed Cobalt Chloride treated HeLa cells (left) and untreaed cell (right) using 20960-1-AP (HIF1a antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
WB analysis using 20960-1-AP
Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 20960-1-AP (HIF1a Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human thyroid cancer using 20960-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 20960-1-AP (HIF1A antibody) at dilution of 1:50 (under 40x lens).
IP experiment of HeLa using 20960-1-AP
IP Result of anti-HIF1a (IP:20960-1-AP, 4ug; Detection:20960-1-AP 1:300) with HeLa cells lysate 4000ug.
FC experiment of HeLa using 20960-1-AP
1X10^6 HeLa cells were stained with 0.2ug HIF1a antibody (20960-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Cat No. 20960-1-AP

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Tested Applications

Positive WB detected in	Cobalt Chloride treated HeLa cells, HeLa cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human thyroid cancer tissue, mouse kidney tissue, human heart tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	Cobalt Chloride treated HeLa cells
Positive FC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 20 publications below
WB	See 160 publications below
IHC	See 38 publications below
IF	See 20 publications below
IP	See 2 publications below
CoIP	See 1 publications below
ChIP	See 2 publications below

Product Information

The immunogen of 20960-1-AP is HIF1a Fusion Protein expressed in E. coli.

Tested Reactivity	human
Cited Reactivity	chicken, human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HIF1a fusion protein Ag15198
Full Name	hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Calculated molecular weight	826 aa, 93 kDa
Observed molecular weight	120 kDa
GenBank accession number	BC012527
Gene symbol	HIF1A
Gene ID (NCBI)	3091
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .

Protocols

Product Specific Protocols
WB protocol for HIF1a antibody 20960-1-AP	Download protocol
IHC protocol for HIF1a antibody 20960-1-AP	Download protocol
IF protocol for HIF1a antibody 20960-1-AP	Download protocol
IP protocol for HIF1a antibody 20960-1-AP	Download protocol
FC protocol for HIF1a antibody 20960-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Coordinative metabolism of glutamine carbon and nitrogen in proliferating cancer cells under hypoxia. Authors - Yuanyuan Wang View Article
rat	WB	J Physiol Chronic fetal hypoxia disrupts the peri-conceptual environment in next-generation adult female rats. Authors - C E Aiken View Article
human	WB	Front Oncol Targeting JUN, CEBPB, and HDAC3: A Novel Strategy to Overcome Drug Resistance in Hypoxic Glioblastoma. Authors - Yixing Gao View Article
human	WB,IHC	Theranostics Oncogenic MSH6-CXCR4-TGFB1 Feedback Loop: A Novel Therapeutic Target of Photothermal Therapy in Glioblastoma Multiforme. Authors - Yaodong Chen View Article
human	WB	Cancer Lett UHRF1 promotes aerobic glycolysis and proliferation via suppression of SIRT4 in pancreatic cancer. Authors - Qiangsheng Hu View Article
human	WB	Onco Targets Ther Hypoxic non-small-cell lung cancer cell-derived exosomal miR-21 promotes resistance of normoxic cell to cisplatin. Authors - Caijun Dong View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Chun (Verified Customer) (12-05-2019)

A good antibody

Applications: Western Blot,
Primary Antibody Dilution: 1:1,000
Cell Tissue Type: HeLa cells