Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, MCF-7 cells, RAW 264.7 cells, rat skeletal muscle tissue, mouse skeletal muscle tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 12 publications below |
| WB | See 282 publications below |
| IHC | See 45 publications below |
| IF | See 17 publications below |
| IP | See 3 publications below |
| CoIP | See 4 publications below |
Product Information
22029-1-AP targets Hexokinase 2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, canine, monkey, chicken, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Hexokinase 2 fusion protein Ag16895 Predict reactive species |
| Full Name | hexokinase 2 |
| Calculated Molecular Weight | 102 kDa |
| Observed Molecular Weight | 102 kDa |
| GenBank Accession Number | BC021116 |
| Gene Symbol | Hexokinase 2 |
| Gene ID (NCBI) | 3099 |
| RRID | AB_11182717 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P52789 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236). The antibody also recognizes Hexokinase 1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Hexokinase 2 antibody 22029-1-AP | Download protocol |
| IHC protocol for Hexokinase 2 antibody 22029-1-AP | Download protocol |
| IF protocol for Hexokinase 2 antibody 22029-1-AP | Download protocol |
| IP protocol for Hexokinase 2 antibody 22029-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption | ||
Cell Metab An Upstream Open Reading Frame in Phosphatase and Tensin Homolog Encodes a Circuit Breaker of Lactate Metabolism. | ||
Cell Metab STAT3 Activation-Induced Fatty Acid Oxidation in CD8+ T Effector Cells Is Critical for Obesity-Promoted Breast Tumor Growth. | ||
Cell Metab NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis. | ||
Nat Neurosci Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex. | ||
Nat Commun Parvimonas micra promotes oral squamous cell carcinoma metastasis through TmpC-CKAP4 axis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH SONAM (Verified Customer) (02-22-2024) | Works well
|
FH Maialen (Verified Customer) (10-03-2023) | It works very well
 |
FH Macarena (Verified Customer) (10-07-2022) | excellent results. Very specific
|
FH Henar (Verified Customer) (08-31-2022) | Great antibody! No background and very specific result.
|
