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- KD/KO Validated
Hexokinase 2 Polyclonal antibody
Hexokinase 2 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, CoIP,ELISA
Conjugate
Unconjugated
Cat no : 22029-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, HeLa cells, mouse skeletal muscle tissue, Jurkat cells, MCF-7 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human breast cancer tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 4 publications below |
WB | See 69 publications below |
IHC | See 15 publications below |
IF | See 3 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Product Information
The immunogen of 22029-1-AP is Hexokinase 2 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, swine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Hexokinase 2 fusion protein Ag16895 |
Full Name | hexokinase 2 |
Calculated molecular weight | 102 kDa |
Observed molecular weight | 102 kDa |
GenBank accession number | BC021116 |
Gene symbol | HK2 |
Gene ID (NCBI) | 3099 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236)
Protocols
Product Specific Protocols | |
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WB protocol for Hexokinase 2 antibody 22029-1-AP | Download protocol |
IHC protocol for Hexokinase 2 antibody 22029-1-AP | Download protocol |
IP protocol for Hexokinase 2 antibody 22029-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Cancer Oxidized Vitamin C (DHA) Overcomes Resistance to EGFR-targeted Therapy of Lung Cancer through Disturbing Energy Homeostasis. | ||
Contrast Media Mol Imaging Fructose 1,6-Bisphosphatase 1 Expression Reduces 18F-FDG Uptake in Clear Cell Renal Cell Carcinoma. | ||
Cancer Res Retinoic acid-related orphan receptor C regulates proliferation, glycolysis, and chemoresistance via the PD-L1/ITGB6/STAT3 signaling axis in bladder cancer. | ||
Mol Cancer Long non-coding RNA PVT1 promotes tumor progression by regulating the miR-143/HK2 axis in gallbladder cancer.
| ||
Cell Commun Signal Blockade of glycolysis-dependent contraction by oroxylin a via inhibition of lactate dehydrogenase-a in hepatic stellate cells. | ||
Cancer Lett UHRF1 promotes aerobic glycolysis and proliferation via suppression of SIRT4 in pancreatic cancer. |