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HLA-E Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 27411-1-AP

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Con: 50 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

HLA-E Antibody WB human placenta tissue 27411-1-AP

human placenta tissue were subjected to SDS PAGE followed by western blot with 27411-1-AP (HLA-E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HLA-E Antibody IHC human tonsillitis tissue 27411-1-AP

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27411-1-AP( HLA-E antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

HLA-E Antibody IHC human tonsillitis tissue 27411-1-AP

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27411-1-AP( HLA-E antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human
Positive WB detected in:
human placenta tissue, HL-60 cells, U-937 cells
Positive IHC detected in:
human tonsillitis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
HLA-E fusion protein Ag26581
Full name:
major histocompatibility complex, class I, E
Calculated molecular weight:
40 kDa
Observed molecular weight:
42 kDa
GenBank accession number:
BC002578
Gene ID (NCBI):
3133
Gene symbol
HLA-E
Synonyms
EA1.2, EA2.1, HLA 6.2, HLA E, HLAE, HLA-E, MHC, MHC class I antigen E, QA1

Background

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-E is a non-classical MHC class I molecule. HLA-E is frequently overexpressed in tumor diseases, transplants and virus-infected cells and represents an immunomodulatory molecule by binding to the receptors CD94/NKG2A, -B and -C on NK and T cells. Due to its immune suppressive features HLA-E expression might represent an important mechanism of tumors to escape immune surveillance.(PMID: 667938; 3375250; 2249951; 27589686)


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