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WB analysis of THP-1 using 27411-1-AP
WB result of HLA-E antibody (27411-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-HLA-E transfected THP-1 cells.
WB analysis using 27411-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27411-1-AP (HLA-E antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 27411-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 27411-1-AP (HLA-E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 27411-1-AP
MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 27411-1-AP (HLA-E antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 27411-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 27411-1-AP (HLA-E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 27411-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 27411-1-AP (HLA-E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of THP-1 using 27411-1-AP
IP result of anti-HLA-E (IP:27411-1-AP, 4ug; Detection:27411-1-AP 1:4000) with THP-1 cells lysate 1720 ug.
IHC staining of human tonsillitis using 27411-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27411-1-AP (HLA-E antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 27411-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27411-1-AP (HLA-E antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of Raji using 27411-1-AP
Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-E antibody (27411-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

HLA-E Polyclonal antibody

HLA-E Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 27411-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

HLA 6.2, HLA E, HLA-6.2, HLAE, MHC class I antigen E

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	Raji cells, HL-60 cells, human placenta tissue, MDA-MB-231 cells, THP-1 cells, U-937 cells, Ramos cells
Positive IP detected in	THP-1 cells
Positive IHC detected in	human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Raji cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27411-1-AP targets HLA-E in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag26581 Product name: Recombinant human HLA-E protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 72-200 aa of BC002578 Sequence: WMEQEGSEYWDRETRSARDTAQIFRVNLRTLRGYYNQSEAGSHTLQWMHGCELGPDRRFLRGYEQFAYDGKDYLTLNEDLRSWTAVDTAAQISEQKSNDASEAEHQRAYLEDTCVEWLHKYLEKGKETL Predict reactive species
Full Name	major histocompatibility complex, class I, E
Calculated Molecular Weight	40 kDa
Observed Molecular Weight	40-42 kDa
GenBank Accession Number	BC002578
Gene Symbol	HLA-E
Gene ID (NCBI)	3133
RRID	AB_2880865
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P13747
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-E is a non-classical MHC class I molecule. HLA-E is frequently overexpressed in tumor diseases, transplants and virus-infected cells and represents an immunomodulatory molecule by binding to the receptors CD94/NKG2A, -B and -C on NK and T cells. Due to its immune suppressive features HLA-E expression might represent an important mechanism of tumors to escape immune surveillance.(PMID: 667938; 3375250; 2249951; 27589686)

Protocols

Product Specific Protocols
IF protocol for HLA-E antibody 27411-1-AP	Download protocol
IHC protocol for HLA-E antibody 27411-1-AP	Download protocol
IP protocol for HLA-E antibody 27411-1-AP	Download protocol
WB protocol for HLA-E antibody 27411-1-AP	Download protocol