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  • Featured Product
  • KD/KO Validated

HMG20A Polyclonal antibody

HMG20A Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12085-2-AP

Synonyms

high mobility group 20A, HMG box containing protein 20A, HMG20A, HMGX1, HMGXB1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using HMG20A Polyclonal antibody (12085-2-AP)

    WB analysis of HEK-293 using 12085-2-AP

    WB result of HMG20A antibody (12085-2-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-HMG20A transfected HEK-293 cells.

  • Western Blot (WB) analysis of Jurkat cells using HMG20A Polyclonal antibody (12085-2-AP)

    WB analysis of Jurkat using 12085-2-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12085-2-AP (HMG20A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using HMG20A Polyclonal antibody (12085-2-AP)

    WB analysis of Jurkat using 12085-2-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12085-2-AP (HMG20A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using HMG20A Polyclonal antibody (12085-2-AP)

    WB analysis of Jurkat using 12085-2-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12085-2-AP (HMG20A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using HMG20A Polyclonal antibody (12085-2-AP)

    IP experiment of Jurkat using 12085-2-AP

    IP result of anti-HMG20A (IP:12085-2-AP, 4ug; Detection:12085-2-AP 1:500) with Jurkat cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using HMG20A Polyclonal antibody (12085-2-AP)

    IHC staining of human pancreas cancer using 12085-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12085-2-AP (HMG20A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using HMG20A Polyclonal antibody (12085-2-AP)

    IHC staining of human pancreas cancer using 12085-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12085-2-AP (HMG20A antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HMG20A Polyclonal antibody (12085-2-AP)

    IF Staining of HepG2 using 12085-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12085-2-AP (HMG20A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HMG20A Polyclonal antibody (12085-2-AP)

    IF Staining of HepG2 using 12085-2-AP

    Immunofluorescent analysis of HepG2 cells, using HMG20A antibody 12085-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HMG20A Polyclonal antibody (12085-2-AP)

    IF Staining of HepG2 using 12085-2-AP

    Immunofluorescent analysis of HepG2 cells, using HMG20A antibody 12085-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12085-2-AP targets HMG20A in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMG20A fusion protein Ag2718
Full Name high-mobility group 20A
Calculated Molecular Weight 347 aa, 40 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC021959
Gene Symbol HMG20A
Gene ID (NCBI) 10363
RRIDAB_2117587
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize nucleosome formation and acts as a transcription-factor-like protein that regulates the expression of several genes. HMG20A has a role in the initiation of neuronal differentiation by activating REST-responsive genes, and it also nvolves in the recruitment of the histone methyltransferase MLL and consequent increased methylation of histone H3 lysine 4. HMG20A (for inhibitor of BRAF35) overcomes the repressive effects of the neuronal silencer REST and activates REST-responsive genes through the modulation of histone methylation

Protocols

Product Specific Protocols
WB protocol for HMG20A antibody 12085-2-APDownload protocol
IHC protocol for HMG20A antibody 12085-2-APDownload protocol
IF protocol for HMG20A antibody 12085-2-APDownload protocol
IP protocol for HMG20A antibody 12085-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Nat Commun

The H2A.Z and NuRD associated protein HMG20A controls early head and heart developmental transcription programs

Authors - Andreas Herchenröther
  • KD Validated
View Article
humanWB

Nat Commun

KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics

Authors - Mai Abdel Mouti
View Article
humanWB

EMBO Mol Med

LSD1 inhibition induces differentiation and cell death in Merkel cell carcinoma.

Authors - Lukas Leiendecker
View Article
mouseWB

Cancer Cell Int

Circular RNA ABCB10 promotes hepatocellular carcinoma progression by increasing HMG20A expression by sponging miR-670-3p.

Authors - Yu Fu
View Article
humanIHC

Biochim Biophys Acta

Interactome of the inhibitory isoform of the nuclear transporter Importin 13.

Authors - Shadma Fatima
View Article
mouseWB,IHC,IF,IP

Biochim Biophys Acta

The BRCA1-binding protein BRAP2 can act as a cytoplasmic retention factor for nuclear and nuclear envelope-localizing testicular proteins.

Authors - Davies Rebecca G RG
View Article