Name: HMGB1 Rabbit Polyclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IP, IHC, IF, FC, ChIP, ELISA
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Images See all

HMGB1 Antibody WB Jurkat cells 10829-1-AP

WB result of HMGB1 antibody (10829-1-AP, 1:1500) with si-control and si-HMGB1 transfected Jurkat cells.

Jurkat cells were subjected to SDS PAGE followed by western blot with 10829-1-AP(HMGB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HMGB1 Antibody IP HepG2 cells 10829-1-AP

IP Result of anti-HMGB1 (IP:10829-1-AP, 3ug; Detection:10829-1-AP 1:500) with HepG2 cells lysate 2900ug.

HMGB1 Antibody IHC human breast cancer tissue 10829-1-AP

Immunohistochemical of paraffin-embedded human breast cancer using 10829-1-AP(HMGB1 antibody) at dilution of 1:100 (under 10x lens)

Species specificity:

human, mouse, rat

Positive WB detected in:

Jurkat cells, RAW 264.7 cells, human brain tissue, rat stomach tissue, , rat brain tissue, human testis tissue, mouse kidney tissue

Positive IP detected in:

HepG2 cells

Positive IHC detected in:

human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution:

WB : 1:500-1:2000

IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

IHC : 1:50-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

HMGB1 fusion protein Ag1264

Full name:

high-mobility group box 1

Calculated molecular weight:

25 kDa

Observed molecular weight:

30 kDa

GenBank accession number:

BC003378

Gene ID (NCBI):

3146

Gene symbol

HMGB1

Synonyms

high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1, HMG1, HMG3, HMGB1, SBP 1

Background

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize NUCLEOSOME formation and acts as a transcription-factor-like protein that regulates the expression of several genes[PMID: 18160415]. Once injury, infection or other inflammatory stimuli, activated macrophages, mature dendritic cells and natural killer cells can secret HMGB1, which act as a crucial cytokine[PMID: 20163887]. HMGB1 also involved in V(D)J recombination by acting as a cofactor of the RAG complex, stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS)[PMID: 19360789 ]. Act as a Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. HMGB1 (high mobility group box 1) modulates gene expression in the nucleus, but certain immune cells secrete HMGB1 as an extracellular Alarmin to signal tissue damage.The nuclear HMGB1 relocalizes to the extracellular milieu in senescent human and mouse cells in culture and in vivo, which stimulated cytokine secretion through TLR-4 signaling (23649808).This antibody is a rabbit polyclonal antibody raised against full length HMGB1 of human origin. The monomeric HMGB1 is 29 kDa and dimer is 58 kDa.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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HMGB1 Rabbit Polyclonal antibody

PATRICK LINK, 12-01-2018

Applications

Immunofluorescence,

Primary Antibody Dilution

1:200

Cell/Tissue Type

PRIMARY ISOLATED ENDOTHELIAL CELLS

Lot Number

This antibody worked well. ProteinTech provides antibody specific instructions that were helpful for each of the antibodies we ordered.

Showing 1- [6] of [41] Publications

Author:		Xing Guo
Pubmed ID:		27561680
Journal:		Nat Commun
Title:		VCP recruitment to mitochondria causes mitophagy impairment and neurodegeneration in models of Huntington's disease.
Application:		WB

Species:		human

Author:		Davalos Albert R AR
Pubmed ID:		23649808
Journal:		J Cell Biol
Title:		p53-dependent release of Alarmin HMGB1 is a central mediator of senescent phenotypes.
Application:		WB

Species:		human

Author:		Muhammad Riaz Khan
Pubmed ID:		29046333
Journal:		EMBO J
Title:		The p53-inducible long noncoding RNA TRINGS protects cancer cells from necrosis under glucose starvation.
Application:		WB

Species:		human

Author:		Hongmei Liu
Pubmed ID:		27851749
Journal:		PLoS Biol
Title:		The Machado-Joseph Disease Deubiquitinase Ataxin-3 Regulates the Stability and Apoptotic Function of p53.
Application:

Species:

Author:		Yu Miao M
Pubmed ID:		18160415
Journal:		Nucleic Acids Res
Title:		Proteomic screen defines the hepatocyte nuclear factor 1alpha-binding partners and identifies HMGB1 as a new cofactor of HNF1alpha.
Application:		WB,ChIP,IF
KD validated
Species:		human

Author:		Hong Fan
Pubmed ID:		26842216
Journal:		Mol Neurodegener
Title:		Reactive astrocytes undergo M1 microglia/macrohpages-induced necroptosis in spinal cord injury.
Application:		IHC

Species:		mouse

WB Figures

HMGB1 Antibody WB human brain tissue 10829-1-AP

human brain tissue were subjected to SDS PAGE followed by western blot with 10829-1-AP(HMGB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HMGB1 Antibody WB human testis tissue 10829-1-AP

human testis tissue were subjected to SDS PAGE followed by western blot with 10829-1-AP(HMGB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

WB result of HMGB1 antibody (10829-1-AP, 1:1500) with si-control and si-HMGB1 transfected Jurkat cells.

Jurkat cells were subjected to SDS PAGE followed by western blot with 10829-1-AP(HMGB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HMGB1 Antibody WB mouse kidney tissue 10829-1-AP

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10829-1-AP(HMGB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HMGB1 Antibody WB rat brain tissue 10829-1-AP

rat brain tissue were subjected to SDS PAGE followed by western blot with 10829-1-AP( HMGB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

HMGB1 Antibody WB rat stomach tissue 10829-1-AP

rat stomach tissue were subjected to SDS PAGE followed by western blot with 10829-1-AP( HMGB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HMGB1 Antibody WB RAW 264.7 cells 10829-1-AP

RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10829-1-AP (HMGB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemical of paraffin-embedded human breast cancer using 10829-1-AP(HMGB1 antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human breast cancer using 10829-1-AP(HMGB1 antibody) at dilution of 1:50 (under 10x lens)

IP Figures

IP Result of anti-HMGB1 (IP:10829-1-AP, 3ug; Detection:10829-1-AP 1:500) with HepG2 cells lysate 2900ug.

HMGB1 Antibody 41 Publications

Rabbit Polyclonal| Catalog number: 10829-1-AP

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background

Customer Reviews

HMGB1 Rabbit Polyclonal antibody

Showing 1- [6] of [41] Publications

WB Figures

IHC Figures

IP Figures

HMGB1 Antibody 41 Publications

Rabbit Polyclonal| Catalog number: 10829-1-AP

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background

Customer Reviews

HMGB1 Rabbit Polyclonal antibody