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HMGB1 Antibody 39 Publications

Rabbit Polyclonal| Catalog number: 10829-1-AP

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Con: 36 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ChIP, ELISA
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Images See all

HMGB1 Antibody WB Jurkat cells 10829-1-AP

WB result of HMGB1 antibody (10829-1-AP, 1:1500) with si-control and si-HMGB1 transfected Jurkat cells.

HMGB1 Antibody WB Jurkat cells 10829-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 10829-1-AP(HMGB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HMGB1 Antibody IP HepG2 cells 10829-1-AP

IP Result of anti-HMGB1 (IP:10829-1-AP, 3ug; Detection:10829-1-AP 1:500) with HepG2 cells lysate 2900ug.

HMGB1 Antibody IHC human breast cancer tissue 10829-1-AP

Immunohistochemical of paraffin-embedded human breast cancer using 10829-1-AP(HMGB1 antibody) at dilution of 1:100 (under 10x lens)

Species specificity:
human, mouse, rat
Positive WB detected in:
Jurkat cells, RAW 264.7 cells, human brain tissue, rat stomach tissue, , rat brain tissue, human testis tissue, mouse kidney tissue
Positive IP detected in:
HepG2 cells
Positive IHC detected in:
human breast cancer tissue
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
HMGB1 fusion protein Ag1264
Full name:
high-mobility group box 1
Calculated molecular weight:
25 kDa
Observed molecular weight:
30 kDa
GenBank accession number:
BC003378
Gene ID (NCBI):
3146
Gene symbol
HMGB1
Synonyms
high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1, HMG1, HMG3, HMGB1, SBP 1

Background

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize NUCLEOSOME formation and acts as a transcription-factor-like protein that regulates the expression of several genes[PMID: 18160415]. Once injury, infection or other inflammatory stimuli, activated macrophages, mature dendritic cells and natural killer cells can secret HMGB1, which act as a crucial cytokine[PMID: 20163887]. HMGB1 also involved in V(D)J recombination by acting as a cofactor of the RAG complex, stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS)[PMID: 19360789 ]. Act as a Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. HMGB1 (high mobility group box 1) modulates gene expression in the nucleus, but certain immune cells secrete HMGB1 as an extracellular Alarmin to signal tissue damage.The nuclear HMGB1 relocalizes to the extracellular milieu in senescent human and mouse cells in culture and in vivo, which stimulated cytokine secretion through TLR-4 signaling (23649808).This antibody is a rabbit polyclonal antibody raised against full length HMGB1 of human origin. The monomeric HMGB1 is 29 kDa and dimer is 58 kDa.


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