HMOX2 Antibody

HMOX2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14817-1-AP

Synonyms

heme oxygenase (decycling) 2, Heme oxygenase 2, HMOX2, HO 2, HO2



Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, mouse spleen tissue, Raji cells
Positive IP detected inRaji cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14817-1-AP targets HMOX2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMOX2 fusion protein Ag6575
Full Name heme oxygenase (decycling) 2
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession number BC002396
Gene symbol HMOX2
Gene ID (NCBI) 3163
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Heme oxygenase is the rate-limiting enzyme in the heme degradative pathway. There are 2 isozymic forms of heme oxygenase, an inducible heme oxygenase-1 (HMOX1) and a constitutive heme oxygenase-2 (HMOX2). Down-regulation of HMOX2 is associated with the increased expression of HMOX1 in human cell lines. HMOX2 is also named as HO-2 and could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.

Protocols

Product Specific Protocols
WB protocol for HMOX2 antibody 14817-1-APDownload protocol
IHC protocol for HMOX2 antibody 14817-1-APDownload protocol
IF protocol for HMOX2 antibody 14817-1-APDownload protocol
IP protocol for HMOX2 antibody 14817-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

PLoS One

Carbon Monoxide (CO) Released from Tricarbonyldichlororuthenium (II) Dimer (CORM-2) in Gastroprotection against Experimental Ethanol-Induced Gastric Damage.

Authors - Katarzyna Magierowska
human,mouseWB,IHC

Oxid Med Cell Longev

Hydrogen Sulfide Abrogates Hemoglobin-Lipid Interaction in Atherosclerotic Lesion.

Authors - László Potor
humanWB

J Cell Sci

Role of epithelial sodium channels (ENaCs) in endothelial function.

Authors - Dongqing Guo