Tested Applications
| Positive WB detected in | HaCaT cells, hTERT-RPE1 cells, mouse spleen tissue, rat spleen tissue |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14817-1-AP targets HMOX2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6575 Product name: Recombinant human HMOX2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-293 aa of BC002396 Sequence: MSAEVETSEGVDESEKKNSGALEKENQMRMADLSELLKEGTKEAHDRAENTQFVKDFLKGNIKKELFKLATTALYFTYSALEEEMERNKDHPAFAPLYFPMELHRKEALTKDMEYFFGENWEEQVQCPKAAQKYVERIHYIGQNEPELLVAHAYTRYMGDLSGGQVLKKVAQRALKLPSTGEGTQFYLFENVDNAQQFKQLYRARMNALDLNMKTKERIVEEANKAFEYNMQIFNELDQAGSTLARETLEDGFPVHDGKGDMRKCPFYAAEQDKGALEGSSCPFRTAMAVLRK Predict reactive species |
| Full Name | heme oxygenase (decycling) 2 |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC002396 |
| Gene Symbol | HMOX2 |
| Gene ID (NCBI) | 3163 |
| RRID | AB_2118843 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30519 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Heme oxygenase is the rate-limiting enzyme in the heme degradative pathway. There are 2 isozymic forms of heme oxygenase, an inducible heme oxygenase-1 (HMOX1) and a constitutive heme oxygenase-2 (HMOX2). Down-regulation of HMOX2 is associated with the increased expression of HMOX1 in human cell lines. HMOX2 is also named as HO-2 and could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HMOX2 antibody 14817-1-AP | Download protocol |
| IHC protocol for HMOX2 antibody 14817-1-AP | Download protocol |
| IP protocol for HMOX2 antibody 14817-1-AP | Download protocol |
| WB protocol for HMOX2 antibody 14817-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HMOX2 Polyclonal antibody (Cat# 14817-1-AP) has 13 citations published in journals including Nature, Molecular Cell, Acta Pharmaceutica Sinica B, Cell Death & Disease, Free Radical Biology and Medicine, iScience, Oxidative Medicine and Cellular Longevity, Journal of Cell Science, Cells, Renal Failure, American Journal of Physiology, International Journal of Urology, and PLoS ONE. Research fields span heme metabolism, carbon monoxide signaling, ferroptosis, necroptosis, gastric protection, atherosclerosis, diabetic kidney disease, and mitochondrial biology.
| Species | Application | Title |
|---|---|---|
Mol Cell Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability. | ||
Acta Pharm Sin B Organic carbon monoxide prodrug, BW-CO-111, in protection against chemically-induced gastric mucosal damage. | ||
Cell Death Dis Identification of human MLKL Cys184 and HSPBP1 Cys201 as novel cellular targets for necroptosis. | ||
Free Radic Biol Med Heme-mediated liver-heart axis promotes myocardial pyroptosis in db/db mice. | ||
Cells Evidence for Cytoprotective Effect of Carbon Monoxide Donor in the Development of Acute Esophagitis Leading to Acute Esophageal Epithelium Lesions. |
