HNRNPU Polyclonal antibody

HNRNPU Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 14599-1-AP

Synonyms

hnRNP U, HNRNPU, HNRPU, p120, pp120, SAF A, SAFA, Scaffold attachment factor A, U21.1



Tested Applications

Positive WB detected inHEK-293 cells, mouse lung tissue, HeLa cells, Jurkat cells, K-562 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14599-1-AP targets HNRNPU in WB, RIP, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNRNPU fusion protein Ag6141
Full Name heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
Calculated Molecular Weight 825 aa, 91 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC003621
Gene Symbol HNRNPU
Gene ID (NCBI) 3192
RRIDAB_2248577
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HNRNPU, also named as HNRPU, SAFA, and U21.1, is a 825 amino acid protein, which is extensively phosphorylated. HNRNPU as a component of ribonucleosomes localized in cytoplasmic mRNP granules containing untranslated mRNAs. HNRNPU is a component of the CRD-mediated complex that promotes MYC mRNA stabilization. HNRNPU Plays a role in the circadian regulation of the core clock component ARNTL/BMAL1 transcription. The calculated molecular weight of HNRNPU is 90 kDa, but the 120 kDa HNRNPU protein is an abundant nucleoplasmic phosphoprotein that can be crosslinked to pre-mRNA in intact cells by UV light (PMID: 1628625).

Protocols

Product Specific Protocols
WB protocol for HNRNPU antibody 14599-1-APDownload protocol
IF protocol for HNRNPU antibody 14599-1-APDownload protocol
IP protocol for HNRNPU antibody 14599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

Authors - Shaojun Zhang
humanWB,IP

Nucleic Acids Res

p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway

Authors - Xinxing Zhu
WB

Nat Commun

Profiling PRMT methylome reveals roles of hnRNPA1 arginine methylation in RNA splicing and cell growth.

Authors - Wen-Juan Li
humanWB,RIP

Cancer Res

DIS3L2 promotes progression of hepatocellular carcinoma via hnRNP U-mediated alternative splicing.

Authors - Songge Xing
humanWB

Elife

Human DUX4 and mouse Dux interact with STAT1 and broadly inhibit interferon-stimulated gene induction

Authors - Amy E Spens
humanWB

Hum Mol Genet

Nuclear TDP-43 causes neuronal toxicity by escaping from the inhibitory regulation by hnRNPs.

Authors - Hiroaki Suzuki