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HSBP1 Polyclonal antibody

HSBP1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10169-2-AP

Synonyms

HSBP1, HSF1BP

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using HSBP1 Polyclonal antibody (10169-2-AP)

    WB analysis of mouse brain using 10169-2-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10169-2-AP (HSBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HSBP1 Polyclonal antibody (10169-2-AP)

    WB analysis using 10169-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10169-2-AP (HSBP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using HSBP1 Polyclonal antibody (10169-2-AP)

    WB analysis of human brain using 10169-2-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10169-2-AP (HSBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using HSBP1 Polyclonal antibody (10169-2-AP)

    WB analysis of HT-1080 using 10169-2-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 10169-2-AP (HSBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using HSBP1 Polyclonal antibody (10169-2-AP)

    WB analysis of mouse brain using 10169-2-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10169-2-AP (HSBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using HSBP1 Polyclonal antibody (10169-2-AP)

    IP experiment of mouse brain using 10169-2-AP

    IP result of anti-HSBP1 (IP:10169-2-AP, 4ug; Detection:10169-2-AP 1:300) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HSBP1 Polyclonal antibody (10169-2-AP)

    IHC staining of human prostate cancer using 10169-2-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10169-2-AP (HSBP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using HSBP1 Polyclonal antibody (10169-2-AP)

    IHC staining of mouse liver using 10169-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10169-2-AP (HSBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using HSBP1 Polyclonal antibody (10169-2-AP)

    IHC staining of mouse liver using 10169-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10169-2-AP (HSBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, HT-1080 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse liver tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10169-2-AP targets HSBP1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSBP1 fusion protein Ag0225
Full Name heat shock factor binding protein 1
Calculated Molecular Weight 8.5 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC007515
Gene Symbol HSBP1
Gene ID (NCBI) 3281
RRIDAB_2119501
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HSBP1 antibody 10169-2-APDownload protocol
IHC protocol for HSBP1 antibody 10169-2-APDownload protocol
IP protocol for HSBP1 antibody 10169-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Endocrinol

Blocking the interaction between circTNRC18 and LIN28A promotes trophoblast epithelial-mesenchymal transformation and alleviates preeclampsia

Authors - Li-Li Chen
View Article