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HSD17B6 Polyclonal antibody

HSD17B6 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC

Conjugate

Unconjugated

Cat no : 11855-1-AP

Synonyms

17 beta HSD 6, 17 beta HSD6, 3 alpha >beta HSE, HSD17B6, HSE, RODH, SDR9C6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSD17B6 Polyclonal antibody (11855-1-AP)

    WB analysis using 11855-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11855-1-AP (HSD17B6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using HSD17B6 Polyclonal antibody (11855-1-AP)

    WB analysis of human liver using 11855-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HSD17B6 Polyclonal antibody (11855-1-AP)

    IHC staining of human lung cancer using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HSD17B6 Polyclonal antibody (11855-1-AP)

    IHC staining of human lung cancer using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using HSD17B6 Polyclonal antibody (11855-1-AP)

    IHC staining of human liver using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11855-1-AP (HSD17B6 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using HSD17B6 Polyclonal antibody (11855-1-AP)

    IHC staining of human liver using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11855-1-AP (HSD17B6 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using HSD17B6 Polyclonal antibody (11855-1-AP)

    IHC staining of human prostate hyperplasia using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11855-1-AP (HSD17B6 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using HSD17B6 Polyclonal antibody (11855-1-AP)

    IHC staining of human prostate hyperplasia using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11855-1-AP (HSD17B6 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HSD17B6 Polyclonal antibody (11855-1-AP)

    IHC staining of human lung cancer using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HSD17B6 Polyclonal antibody (11855-1-AP)

    IHC staining of human lung cancer using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue, human liver tissue, rat liver tissue
Positive IHC detected inhuman lung cancer tissue, human prostate hyperplasia tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11855-1-AP targets HSD17B6 in WB, IHC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSD17B6 fusion protein Ag2427
Full Name hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse)
Calculated Molecular Weight 317 aa, 36 kDa
Observed Molecular Weight 33-35 kDa
GenBank Accession NumberBC020710
Gene Symbol HSD17B6
Gene ID (NCBI) 8630
RRIDAB_2295487
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HSD17B6 antibody 11855-1-APDownload protocol
IHC protocol for HSD17B6 antibody 11855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Breast Cancer Res Treat

The presence and impact of estrogen metabolism on the biology of triple-negative breast cancer.

Authors - Keely May McNamara
View Article
humanWB

Cancer Cell Int

Downexpression of HSD17B6 correlates with clinical prognosis and tumor immune infiltrates in hepatocellular carcinoma.

Authors - Lei Lv
View Article
humanWB

Cell Death Discov

HSD17B6 downregulation predicts poor prognosis and drives tumor progression via activating Akt signaling pathway in lung adenocarcinoma

Authors - Tian Tian
View Article