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HSPA2 Polyclonal antibody

Name: HSPA2 Polyclonal antibody
Brand: Proteintech
SKU: 12797-1-AP

HSPA2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12797-1-AP

Synonyms

Heat shock 70 kDa protein 2, heat shock 70kDa protein 2, HSP70 2, HSP70 3, HSPA2

Validation Data Gallery

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AllWBIPIHCFC

WB analysis of HeLa using 12797-1-AP
WB result of HSPA2 antibody (12797-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPA2 transfected HeLa cells.
WB analysis using 12797-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12797-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 12797-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 12797-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse skeletal muscle using 12797-1-AP
IP result of anti-HSPA2 (IP:12797-1-AP, 4ug; Detection:12797-1-AP 1:1000) with mouse skeletal muscle tissue lysate 4000ug.
IHC staining of human testis using 12797-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 12797-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human prostate hyperplasia using 12797-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human prostate hyperplasia using 12797-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human ovary tumor using 12797-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 12797-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse ovary using 12797-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse ovary using 12797-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of HeLa using 12797-1-AP
1X10^6 HeLa cells were stained with 0.2ug HSPA2 antibody (12797-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Cat No. 12797-1-AP

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Tested Applications

Positive WB detected in	A431 cells, human brain tissue, human kidney tissue, human skeletal muscle tissue, HeLa cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, C6 cells, mouse skeletal tissue
Positive IP detected in	mouse skeletal muscle tissue
Positive IHC detected in	human testis tissue, human prostate hyperplasia tissue, human ovary tumor tissue, mouse ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Flow Cytometry (FC)	FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 7 publications below
IHC	See 6 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

12797-1-AP targets HSPA2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HSPA2 fusion protein Ag3539
Full Name	heat shock 70kDa protein 2
Calculated Molecular Weight	639 aa, 70 kDa
Observed Molecular Weight	70 kDa
GenBank Accession Number	BC036107
Gene Symbol	HSPA2
Gene ID (NCBI)	3306
RRID	AB_2119687
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)

Protocols

Product Specific Protocols
WB protocol for HSPA2 antibody 12797-1-AP	Download protocol
IHC protocol for HSPA2 antibody 12797-1-AP	Download protocol
IP protocol for HSPA2 antibody 12797-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Sci Adv Circular RNAs from BOULE play conserved roles in protection against stress-induced fertility decline. Authors - Liuze Gao View Article
human	IHC	Cell Death Differ RNF144A functions as a tumor suppressor in breast cancer through ubiquitin ligase activity-dependent regulation of stability and oncogenic functions of HSPA2. Authors - Yin-Long Yang KD Validated View Article
human	WB	Curr Biol RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice. Authors - Long Jiang View Article
		J Mol Cell Biol Cigarette smoke-induced cell cycle arrest in spermatocytes [GC-2spd(ts)] is mediated through cross talk between Ahr-Nrf2 pathway and MAPK signaling. Authors - Prabagaran Esakky View Article
mouse	WB,IHC	Development Mutation of Eif4g3, encoding a eukaryotic translation initiation factor, causes male infertility and meiotic arrest of mouse spermatocytes. Authors - Sun Fengyun F View Article
human	WB, IHC	Int J Mol Sci Various Anti-HSPA2 Antibodies Yield Different Results in Studies on Cancer-Related Functions of Heat Shock Protein A2. Authors - Dorota Scieglinska KD Validated View Article

HSPA2 Polyclonal antibody

HSPA2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis of HeLa using 12797-1-AP

WB analysis using 12797-1-AP

WB analysis of human brain using 12797-1-AP

WB analysis of human kidney using 12797-1-AP

WB analysis of human skeletal muscle using 12797-1-AP

IP experiment of mouse skeletal muscle using 12797-1-AP

IHC staining of human testis using 12797-1-AP

IHC staining of human testis using 12797-1-AP

IHC staining of human prostate hyperplasia using 12797-1-AP

IHC staining of human prostate hyperplasia using 12797-1-AP

IHC staining of human ovary tumor using 12797-1-AP

IHC staining of human ovary tumor using 12797-1-AP

IHC staining of mouse ovary using 12797-1-AP

IHC staining of mouse ovary using 12797-1-AP

FC experiment of HeLa using 12797-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Circular RNAs from BOULE play conserved roles in protection against stress-induced fertility decline.

RNF144A functions as a tumor suppressor in breast cancer through ubiquitin ligase activity-dependent regulation of stability and oncogenic functions of HSPA2.

RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.

Cigarette smoke-induced cell cycle arrest in spermatocytes [GC-2spd(ts)] is mediated through cross talk between Ahr-Nrf2 pathway and MAPK signaling.

Mutation of Eif4g3, encoding a eukaryotic translation initiation factor, causes male infertility and meiotic arrest of mouse spermatocytes.

Various Anti-HSPA2 Antibodies Yield Different Results in Studies on Cancer-Related Functions of Heat Shock Protein A2.