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  • KD/KO Validated

HSP27 Polyclonal antibody

HSP27 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 18284-1-AP

Synonyms

28 kDa heat shock protein, Heat shock 27 kDa protein, heat shock 27kDa protein 1, Heat shock protein beta 1, HSP 27, Hsp25, HSP27, HSP28, HSPB1, SRP27, Stress responsive protein 27



Tested Applications

Positive WB detected inA549 cells, HeLa cells, C6 cells, mouse liver tissue, HepG2 cells, U2OS cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, MCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

18284-1-AP targets HSP27 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, rat, fish, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSP27 fusion protein Ag13161
Full Name heat shock 27kDa protein 1
Calculated molecular weight 19-23 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC012768
Gene symbol HSPB1
Gene ID (NCBI) 3315
RRIDAB_2295540
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSPB1, also known as heat shock protein 27 (HSP27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma. In addition to the predicted 27 kDa, an extra 50-55 kDa representing dimeric form of HSPB1 may also be observed (PMID: 21353161). It's notable that mouse HSP27 is also named HSP25 and has the predicted MW between 19-23 kDa.

Protocols

Product Specific Protocols
WB protocol for HSP27 antibody 18284-1-APDownload protocol
IHC protocol for HSP27 antibody 18284-1-APDownload protocol
IF protocol for HSP27 antibody 18284-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Allergy

ACE2 downregulation in olfactory mucosa: Eosinophilic rhinosinusitis as COVID-19 protective factor?

Authors - Concepció Marin

Autophagy

ATG4B/autophagin-1 regulates intestinal homeostasis and protects mice from experimental colitis.

Authors - Cabrera Sandra S
ratIF

J Cell Biol

Human myocytes are protected from titin aggregation-induced stiffening by small heat shock proteins.

Authors - Sebastian Kötter
humanWB,CoIP

Cell Rep

Analysis of proteome-wide degradation dynamics in ALS SOD1 iPSC-derived patient neurons reveals disrupted VCP homeostasis

Authors - Konstantinos Tsioras
humanWB

Antioxid Redox Signal

Peroxiredoxin 6 is a crucial factor in the initial step of mitochondrial clearance and is upstream of the PINK1-Parkin pathway.

Authors - Shuaipeng Ma
humanWB,IHC

CNS Neurosci Ther

FHOD1 is upregulated in glioma cells and attenuates ferroptosis of glioma cells by targeting HSPB1 signaling

Authors - Fan Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tom (Verified Customer) (09-28-2020)

HEK293T lysate (10ug) ran on 4-20% gradient gel. Blocking in 5% BSA for 1 hour, followed by HSP27 (1:1000 in block) overnight at 4 degrees. Goat anti-rabbit HRP (1:10,000) for 1 hour in block.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293
HSP27 Antibody Western Blot validation (1:1000 dilution) in HEK293 (Cat no:18284-1-AP)