|Positive WB detected in||HeLa cells, mouse liver tissue, C6 cells, HepG2 cells|
|Positive IHC detected in||human lung cancer tissue, human liver cancer tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18284-1-AP targets HSP27 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.
|Tested Reactivity||human, rat, mouse|
|Cited Reactivity||fish, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HSP27 fusion protein Ag13161|
|Full Name||heat shock 27kDa protein 1|
|Calculated molecular weight||19-23 kDa|
|Observed molecular weight||24-27 kDa|
|GenBank accession number||BC012768|
|Gene ID (NCBI)||3315|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HSPB1, also known as heat shock protein 27 (HSP27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma. In addition to the predicted 27 kDa, an extra 50-55 kDa representing dimeric form of HSPB1 may also be observed (PMID: 21353161). It's notable that mouse HSP27 is also named HSP25 and has the predicted MW between 19-23 kDa.
Truncated HSPB1 causes axonal neuropathy and impairs tolerance to unfolded protein stress.
Gastroenterol Res Pract
Effects of Ethyl Pyruvate on Bile Duct Ligation-Induced Liver Fibrosis by Regulating Nrf2 Pathway and Proinflammatory Cytokines in Rats.
Biochem Biophys Res Commun
Identification of heat shock protein 27 as a novel autoantigen of Behçet's disease.
Int J Mol Sci
Upregulation of Phosphorylated HSP27, PRDX2, GRP75, GRP78 and GRP94 in Acquired Middle Ear Cholesteatoma Growth.
Chem Biol Interact
Proteomic analysis of apoptosis induction by lariciresinol in human HepG2 cells.
Mol Reprod Dev
Identification of Hsf1 as a novel androgen receptor-regulated gene in mouse sertoli cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tom (Verified Customer) (09-28-2020)
HEK293T lysate (10ug) ran on 4-20% gradient gel. Blocking in 5% BSA for 1 hour, followed by HSP27 (1:1000 in block) overnight at 4 degrees. Goat anti-rabbit HRP (1:10,000) for 1 hour in block.