HSP27 Polyclonal antibody

HSP27 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18284-1-AP

Synonyms

Heat shock protein beta 1, HSP 27, Hsp25, HSP27, HSP28, HSPB1



Tested Applications

Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18284-1-AP targets HSP27 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSP27 fusion protein Ag13161
Full Name heat shock 27kDa protein 1
Calculated molecular weight 19-23 kDa
Observed molecular weight 24-27 kDa
GenBank accession numberBC012768
Gene symbol HSPB1
Gene ID (NCBI) 3315
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPB1, also known as heat shock protein 27 (HSP27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma. In addition to the predicted 27 kDa, an extra 50-55 kDa representing dimeric form of HSPB1 may also be observed (PMID: 21353161). It's notable that mouse HSP27 is also named HSP25 and has the predicted MW between 19-23 kDa.

Protocols

Product Specific Protocols
WB protocol for HSP27 antibody 18284-1-APDownload protocol
IHC protocol for HSP27 antibody 18284-1-APDownload protocol
IF protocol for HSP27 antibody 18284-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

BBA Clin

Truncated HSPB1 causes axonal neuropathy and impairs tolerance to unfolded protein stress.

Authors - Emil Ylikallio
ratIHC

Gastroenterol Res Pract

Effects of Ethyl Pyruvate on Bile Duct Ligation-Induced Liver Fibrosis by Regulating Nrf2 Pathway and Proinflammatory Cytokines in Rats.

Authors - Yonghua Zong
humanWB

Biochem Biophys Res Commun

Identification of heat shock protein 27 as a novel autoantigen of Behçet's disease.

Authors - Peng Chen
humanWB

Int J Mol Sci

Upregulation of Phosphorylated HSP27, PRDX2, GRP75, GRP78 and GRP94 in Acquired Middle Ear Cholesteatoma Growth.

Authors - Ho Kuen Yao KY

Chem Biol Interact

Proteomic analysis of apoptosis induction by lariciresinol in human HepG2 cells.

Authors - Zhan-Jun Ma
mouseWB

Mol Reprod Dev

Identification of Hsf1 as a novel androgen receptor-regulated gene in mouse sertoli cells.

Authors - Lihua Yang