HSP60 Monoclonal antibody
HSP60 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
2F10E7
Cat no : 66041-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | ROS1728 cells, HeLa cells, HEK-293 cells, Jurkat cells, mouse brain tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human ovary tumor tissue, human skin cancer tissue, human spleen tissue, human tonsillitis tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HeLa cells, A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:2000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 6 publications below |
| IF | See 3 publications below |
| ELISA | See 1 publications below |
Product Information
The immunogen of 66041-1-Ig is HSP60 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, swine |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | HSP60 fusion protein Ag7848 |
| Full Name | heat shock 60kDa protein 1 (chaperonin) |
| Calculated molecular weight | 61 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC003030 |
| Gene symbol | HSPD1 |
| Gene ID (NCBI) | 3329 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HSP60 antibody 66041-1-Ig | Download protocol |
| IHC protocol for HSP60 antibody 66041-1-Ig | Download protocol |
| IF protocol for HSP60 antibody 66041-1-Ig | Download protocol |
| IP protocol for HSP60 antibody 66041-1-Ig | Download protocol |
| FC protocol for HSP60 antibody 66041-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Macrophage achieves self-protection against oxidative stress-induced ageing through the Mst-Nrf2 axis. | ||
Neurotoxicology Tetrachlorobenzoquinone triggers the cleavage of Bid and promotes the cross-talk of extrinsic and intrinsic apoptotic signalings in pheochromocytoma (PC) 12 cells. | ||
Cells Brain-Specific Serine-47 Modification of Cytochrome c Regulates Cytochrome c Oxidase Activity Attenuating ROS Production and Cell Death: Implications for Ischemia/Reperfusion Injury and Akt Signaling. | ||
J Neuroinflammation DJ-1 exerts anti-inflammatory effects and regulates NLRX1-TRAF6 via SHP-1 in stroke. | ||
