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Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, pig brain, rabbit brain, chicken brain |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human ovary tumor tissue, human spleen tissue, human tonsillitis tissue, human skin cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells, Neuro-2a cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 33 publications below |
| IF | See 27 publications below |
| IP | See 2 publications below |
| ELISA | See 1 publications below |
Product Information
66041-1-Ig targets HSP60 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken, yeast |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | HSP60 fusion protein Ag7848 Predict reactive species |
| Full Name | heat shock 60kDa protein 1 (chaperonin) |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC003030 |
| Gene Symbol | HSP60 |
| Gene ID (NCBI) | 3329 |
| RRID | AB_11041709 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P10809 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HSP60 antibody 66041-1-Ig | Download protocol |
| IHC protocol for HSP60 antibody 66041-1-Ig | Download protocol |
| IF protocol for HSP60 antibody 66041-1-Ig | Download protocol |
| IP protocol for HSP60 antibody 66041-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Med The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. | ||
Nat Immunol Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity. | ||
J Clin Invest ATM inhibition enhances cancer immunotherapy by promoting mtDNA leakage/cGAS-STING activation. | ||
Autophagy Increased mitophagy protects cochlear hair cells from aminoglycoside-induced damage. | ||
Autophagy Mycobacterium bovis induces mitophagy to suppress host xenophagy for its intracellular survival. |
