HSP60 Monoclonal antibody

HSP60 Monoclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG1


human, mouse, rat and More (1)







Cat no : 66041-1-Ig


Heat shock protein 60, HSP 60, HSP60, HSP65, HSPD1

Tested Applications

Positive WB detected inROS1728 cells, HeLa cells, HEK-293 cells, Jurkat cells, mouse brain tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman ovary tumor tissue, human skin cancer tissue, human spleen tissue, human tonsillitis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells, A549 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66041-1-Ig is HSP60 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSP60 fusion protein Ag7848
Full Name heat shock 60kDa protein 1 (chaperonin)
Calculated molecular weight 61 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC003030
Gene symbol HSPD1
Gene ID (NCBI) 3329
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)


Product Specific Protocols
WB protocol for HSP60 antibody 66041-1-IgDownload protocol
IHC protocol for HSP60 antibody 66041-1-IgDownload protocol
IF protocol for HSP60 antibody 66041-1-IgDownload protocol
IP protocol for HSP60 antibody 66041-1-IgDownload protocol
FC protocol for HSP60 antibody 66041-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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