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HSP60 Antibody 4 Publications

Mouse Monoclonal| Catalog number: 66041-1-Ig |CloneNo.: 2F10E7

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Con: 142 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

HSP60 Antibody IF Hela cells 66041-1-Ig

Immunofluorescent analysis of Hela cells using 66041-1-Ig (HSP60 antibody) at dilution of 1:50 and Rhodamine-Goat antiMouse IgG

HSP60 Antibody WB ROS1728 cells 66041-1-Ig

Western blot analysis of HSP60 in various tissues and cell lines with 66041-1-Ig (HSP60 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours

HSP60 Antibody IP mouse liver tissue 66041-1-Ig

IP Result of anti-HSP60 (IP:66041-1-Ig, 5ug; Detection:66041-1-Ig 1:2000) with mouse liver tissue lysate 4000ug.

HSP60 Antibody IHC human ovary tumor tissue 66041-1-Ig

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66041-1-Ig( HSP60 antibody at dilution of 1:500 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

HSP60 Antibody IHC human ovary tumor tissue 66041-1-Ig

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66041-1-Ig( HSP60 antibody at dilution of 1:500 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

HSP60 Antibody FC HeLa cells 66041-1-Ig

1X10^6 HeLa cells were stained with 0.2ug HSP60 antibody (66041-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
ROS1728 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, mouse brain tissue
Positive IP detected in:
mouse liver tissue
Positive IHC detected in:
human ovary tumor tissue, human skin cancer tissue, human spleen tissue, human testis tissue, human tonsil tissue, human tonsillitis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
Hela cells, Neuro-2a cells
Positive FC detected in:
HeLa cells, A549 cells
Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
IHC : 1:50-1:2000
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
HSP60 fusion protein Ag7848
Full name:
heat shock 60kDa protein 1 (chaperonin)
Calculated molecular weight:
61 kDa
Observed molecular weight:
60 kDa
GenBank accession number:
BC003030
Gene ID (NCBI):
3329
Gene symbol
HSPD1
Synonyms
Heat shock protein 60, HSP 60, HSP60, HSP65, HSPD1

Background

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)


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