Human AFP ELISA Kit0 Publications

Catalog number: KE00132

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Freight/Packing £00.00

Size: 09µ/gram

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Product name:

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatants

Assay type:

0.1 pg/mL

15.6-1000 pg/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Human plamsa 86% 75%-100%
Cell culture supernatants 94% 77%-107%

Samplenmean ( pg/mL)SDCV%
1 20 471.8 13.5 2.9
2 20 119.0 3.8 3.2
3 20 28.3 1.6 5.5

Samplenmean ( pg/mL)SDCV%
1 24 419.3 43.3 10.3
2 24 107.5 3.6 3.3
3 24 26.3 1.2 4.4

Product overview:
KE00132 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The AFP ELISA kit is to be used to detect and quantify protein levels of endogenous AFP. The assay recognizes human AFP. A monoclonal antibody specific for AFP has been pre-coated onto the microwells. The AFP protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for AFP is added to detect the captured AFP protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

AFP, Alpha 1 fetoprotein, Alpha fetoglobulin, alpha fetoprotein, FETA, HPAFP

AFP (alpha-fetoprotein) is a major plasma protein found in the fetus while plasma levels decrease rapidly after birth. AFP is one of the earliest markers of the hepatocyte lineage. High AFP concentrations have been correlated with tumor cell growth. Detection of AFP in plasma is important in diagnosis of hepatocellular carcinoma (HCC), stomach cancer and germ cell cancers. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects.

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