Human AFP ELISA Kit
Sensitivity
0.1 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Human
Cat no : KE00132
Synonyms
AFP, Alpha 1 fetoprotein, Alpha fetoglobulin, alpha fetoprotein, FETA, HPAFP
Validation Data Gallery
Product Information
KE00132 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The AFP ELISA kit is to be used to detect and quantify protein levels of endogenous AFP. The assay recognizes human AFP. An antibody specific for AFP has been pre-coated onto the microwells. The AFP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for AFP is added to detect the captured AFP protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
| Product name | Human AFP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plamsa | 86% | 75%-100% |
| Cell culture supernatants | 94% | 77%-107% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 471.8 | 13.5 | 2.9 |
| 2 | 20 | 119.0 | 3.8 | 3.2 |
| 3 | 20 | 28.3 | 1.6 | 5.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 419.3 | 43.3 | 10.3 |
| 2 | 24 | 107.5 | 3.6 | 3.3 |
| 3 | 24 | 26.3 | 1.2 | 4.4 |
Background Information
AFP (alpha-fetoprotein) is a major plasma protein found in the fetus while plasma levels decrease rapidly after birth. AFP is one of the earliest markers of the hepatocyte lineage. High AFP concentrations have been correlated with tumor cell growth. Detection of AFP in plasma is important in diagnosis of hepatocellular carcinoma (HCC), stomach cancer and germ cell cancers. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms | AFP, Alpha 1 fetoprotein, Alpha fetoglobulin, alpha fetoprotein, FETA, HPAFP |