Human Albumin ELISA Kit

Sensitivity

12.0 ng/mL

Range

50-3200 ng/mL

Reacted Species

Human

Cat no : KE00076

Synonyms

ALB, albumin, GIG42, HSA, PRO0883, PRO0903, PRO1341, PRO1708, PRO2044, PRO2619, PRO2675, Serum albumin, UNQ696/PRO1341



Product Information

KE00076 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Albumin ELISA kit is to be used to detect and quantify protein levels of endogenous Albumin. The assay recognizes human Albumin. An antibody specific for Albumin has been pre-coated onto the microwells. The Albumin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for Albumin is added to detect the captured Albumin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Human Albumin ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Urine, Saliva, Human milk
Assay type Sandwich
Sensitivity 12.0 ng/mL
Range 50-3200 ng/mL
Reacted Species Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Urine100%93%-109%
Saliva73%62%-80%
Human milk77%62%-86%
Human plasma85%76%-96%

IntraAssay

Samplenmean ( ng/mL)SDCV%
120280.010.43.7
2201,286.896.87.5
3202,322.0157.06.6

InterAssay

Samplenmean ( ng/mL)SDCV%
124288.016.05.6
2241,170.899.28.5
3242,265.6219.29.7

Background Information

Albumin is the most abundant protein in blood plasma. It is produced by well-differentiated hepatocytes, and plays an important role in maintaining osmotic pressure. The normal range of albumin in plasma is 3.4-5.4 grams per deciliter (g/dL). Decreased levels of serum albumin may be a sign of kidney diseases or liver diseases (like hepatitis and cirrhosis). This kit is used to quantify the albumin level.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms ALB, albumin, GIG42, HSA, PRO0883, PRO0903, PRO1341, PRO1708, PRO2044, PRO2619, PRO2675, Serum albumin, UNQ696/PRO1341