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Human Fetuin-A ELISA Kit1 Publications

Catalog number: KE00097

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Freight/Packing £00.00

Size: 09µ/gram

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Standard curve of KE00097 Human Fetuin-A ELISA Kit

Standard curve of KE00097 Human Fetuin-A ELISA Kit


Product name:
Human Fetuin-A ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatants

Assay type:

0.1 ng/mL

0.313 - 20 ng/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Human plasma 104% 84%-125%
Cell culture supernatants 106% 81%-125%

Samplenmean ( ng/mL)SDCV%
1 20 1.6 0.05 3.2
2 20 4.6 0.12 2.5
3 20 12.6 0.76 6.1

Samplenmean ( ng/mL)SDCV%
1 20 1.5 0.04 2.8
2 20 4.4 0.23 5.2
3 20 12.9 0.48 3.8

Product overview:
KE00097 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Fetuin A ELISA kit is to be used to detect and quantify protein levels of endogenous Fetuin A. The assay recognizes human Fetuin A. An antibody specific for Fetuin A has been pre-coated onto the microwells. The Fetuin A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for Fetuin A is added to detect the captured Fetuin A protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

A2HS, AHS, AHSG, alpha 2 HS glycoprotein, Alpha 2 Z globulin, Ba alpha 2 glycoprotein, FETUA, Fetuin A, HSGA

Fetuin A, also named as Alpha2-HS glycoprotein (AHSG), is a member of cystatin superfamily of protease inhibitors. It is a highly expressed glycoprotein in various fetal tissues whereas it is mainly expressed by the liver in adults. Fetuin A, an extracellular inhibitor of transforming growth factor β, is a profibrogenic stimulus in liver disease. Circulating fetuin A may be a beneficial serum biomarker in the detection of liver and vascular fibrosis progression in patients with non-alcoholic fatty liver disease. It is also involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Fetuin A exerts its effects on the cardiovascular system by two different mechanisms. On the one hand it inhibits insulin signaling and induces insulin resistance contributing to the onset of atherosclerosis and on the other hand it inhibits calcium deposition and protects from vascular calcification.

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