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ICAM-1 Antibody 38 Publications

Rabbit Polyclonal| Catalog number: 10831-1-AP

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Con: 51 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

ICAM-1 Antibody WB Raji cells 10831-1-AP

WB result of ICAM1 antibody (10831-1-AP, 1:1000) with si-Control and si-ICAM1 transfected Raji cells.

ICAM-1 Antibody WB HUVEC cells 10831-1-AP

HUVEC cells were subjected to SDS PAGE followed by western blot with 10831-1-AP (ICAM-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ICAM-1 Antibody IF human lung cancer tissue 10831-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed human lung cancer tissue using 10831-1-AP (ICAM-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ICAM-1 Antibody IHC human lung cancer tissue 10831-1-AP

Immunohistochemical of paraffin-embedded human lung cancer using 10831-1-AP(ICAM-1 antibody) at dilution of 1:50 (under 25x lens)

ICAM-1 Antibody IHC human liver cancer tissue 10831-1-AP

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 10831-1-AP( ICAM-1 Antibody) at dilution of 1:50 (under 40x lens)

ICAM-1 Antibody IP Raji cells 10831-1-AP

IP Result of anti-ICAM-1 (IP:10831-1-AP, 4ug; Detection:10831-1-AP 1:1000) with Raji cells lysate 1320ug.

ICAM-1 Antibody FC Raji cells 10831-1-AP

1X10^6 Raji cells were stained with 0.2ug ICAM-1 antibody (10831-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, bovine
Positive WB detected in:
HUVEC cells, Raji cells, L02 cells
Positive IP detected in:
Raji cells
Positive IHC detected in:
human lung cancer tissue, human liver cancer tissue, human tonsillitis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
human lung cancer tissue
Positive FC detected in:
Raji cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ICAM-1 fusion protein Ag1188
Full name:
intercellular adhesion molecule 1
Calculated molecular weight:
90 kDa
Observed molecular weight:
85-95 kDa
GenBank accession number:
BC015969
Gene ID (NCBI):
3383
Gene symbol
ICAM1
Synonyms
BB2, CD54, ICAM 1, ICAM1, P3.58

Background

Where is ICAM-1 expressed?

Intercellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54) is a transmembrane glycoprotein constitutively expressed at low levels in endothelial cells, pericytes and on some lymphocytes and monocytes1. It is located at the cytoplasmic membrane, with a large extracellular region of mainly hydrophobic amino acids joined to a small transmembrane region and a cytoplasmic tail. It has a molecular weight of 75 to 115 kDa depending on the level of glycosylation.

 What is the function of ICAM-1?

ICAM-1 is important in both innate and adaptive immune responses as an adhesion molecule. Although it is constitutively expressed, in the presence of pro-inflammatory cytokines such as TNFα the endothelial cells are activated and upregulate expression of ICAM-12. In blood vessels lined with endothelial cells, leukocytes that are rolling over the surface are able to bind to ICAM-1 and transmigrate through the endothelial barrier and into the tissue. The initial binding of the leukocytes to ICAM-1 causes a Ca2+ release that initiates endothelial cell contraction and weakening of the intercellular tight junctions3, 4. This protein can be used as an indicator of endothelial activation and of vascular inflammation.

 What is the role of ICAM-1 in disease?

Beyond the role in the immune response, ICAM-1 has also been identified as the target of attachment for the human rhinovirus, the cause of the common cold. Binding of the virus to ICAM-1 causes the viral capsid to uncoat and leads to release of the genetic material5.

  1.  Hubbard, A. K. & Rothlein, R. Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radic. Biol. Med. 28, 1379–86 (2000).

  2. Long, E. O. ICAM-1: getting a grip on leukocyte adhesion. J. Immunol. 186, 5021–3 (2011).

  3. Lawson, C. & Wolf, S. ICAM-1 signaling in endothelial cells. (2009).

  4. Lyck, R. & Enzmann, G. The physiological roles of ICAM-1 and ICAM-2 in neutrophil migration into tissues. Curr. Opin. Hematol. 22, 53–59 (2015).

  5. Xing, L., Casasnovas, J. M. & Cheng, R. H. Structural analysis of human rhinovirus complexed with ICAM-1 reveals the dynamics of receptor-mediated virus uncoating. J. Virol. 77, 6101–7 (2003).



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