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ICAM-1 Polyclonal antibody

ICAM-1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, FC, Blocking assay, ELISA

Conjugate

Unconjugated

Cat no : 10831-1-AP

Synonyms

BB2, CD54, ICAM 1, ICAM1, P3.58



Tested Applications

Positive WB detected inHUVEC cells, Raji cells, L02 cells
Positive IP detected inRaji cells
Positive IHC detected inhuman lung cancer tissue, human tonsillitis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman lung cancer tissue
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10831-1-AP targets ICAM-1 in WB, IP, IHC, IF, FC, Blocking assay, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity bovine, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ICAM-1 fusion protein Ag1188
Full Name intercellular adhesion molecule 1
Calculated molecular weight 90 kDa
Observed molecular weight 85-95 kDa
GenBank accession numberBC015969
Gene symbol ICAM1
Gene ID (NCBI) 3383
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Where is ICAM-1 expressed?

Intercellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54) is a transmembrane glycoprotein constitutively expressed at low levels in endothelial cells, pericytes and on some lymphocytes and monocytes1. It is located at the cytoplasmic membrane, with a large extracellular region of mainly hydrophobic amino acids joined to a small transmembrane region and a cytoplasmic tail. It has a molecular weight of 75 to 115 kDa depending on the level of glycosylation.

 What is the function of ICAM-1?

ICAM-1 is important in both innate and adaptive immune responses as an adhesion molecule. Although it is constitutively expressed, in the presence of pro-inflammatory cytokines such as TNFα the endothelial cells are activated and upregulate expression of ICAM-12. In blood vessels lined with endothelial cells, leukocytes that are rolling over the surface are able to bind to ICAM-1 and transmigrate through the endothelial barrier and into the tissue. The initial binding of the leukocytes to ICAM-1 causes a Ca2+ release that initiates endothelial cell contraction and weakening of the intercellular tight junctions3, 4. This protein can be used as an indicator of endothelial activation and of vascular inflammation.

 What is the role of ICAM-1 in disease?

Beyond the role in the immune response, ICAM-1 has also been identified as the target of attachment for the human rhinovirus, the cause of the common cold. Binding of the virus to ICAM-1 causes the viral capsid to uncoat and leads to release of the genetic material5.

  1.  Hubbard, A. K. & Rothlein, R. Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radic. Biol. Med. 28, 1379–86 (2000).

  2. Long, E. O. ICAM-1: getting a grip on leukocyte adhesion. J. Immunol. 186, 5021–3 (2011).

  3. Lawson, C. & Wolf, S. ICAM-1 signaling in endothelial cells. (2009).

  4. Lyck, R. & Enzmann, G. The physiological roles of ICAM-1 and ICAM-2 in neutrophil migration into tissues. Curr. Opin. Hematol. 22, 53–59 (2015).

  5. Xing, L., Casasnovas, J. M. & Cheng, R. H. Structural analysis of human rhinovirus complexed with ICAM-1 reveals the dynamics of receptor-mediated virus uncoating. J. Virol. 77, 6101–7 (2003).


Protocols

Product Specific Protocols
WB protocol for ICAM-1 antibody 10831-1-APDownload protocol
IHC protocol for ICAM-1 antibody 10831-1-APDownload protocol
IF protocol for ICAM-1 antibody 10831-1-APDownload protocol
IP protocol for ICAM-1 antibody 10831-1-APDownload protocol
FC protocol for ICAM-1 antibody 10831-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Stem Cell Res Ther

A combination of ultrasound-targeted microbubble destruction with transplantation of bone marrow mesenchymal stem cells promotes recovery of acute liver injury.

Authors - Ting Sun
mouseIF

Cell Biol Int

Role of allograft inflammatory factor-1 in the regulation of inflammation and oxidative stress in primary peritoneal mesothelial cells.

Authors - Yinan Zhou
ratWB

Neuroscience

LXA4 ameliorates cerebrovascular endothelial dysfunction by reducing acute inflammation after subarachnoid hemorrhage in rats.

Authors - Liu Liu
ratWB,IHC

Food Chem Toxicol

Aqueous extract of Salvia miltiorrhiza Bunge-Radix Puerariae herb pair ameliorates diabetic vascular injury by inhibiting oxidative stress in streptozotocin-induced diabetic rats.

Authors - Wenwen Zhao
mouseWB

Invest Ophthalmol Vis Sci

Transducin1, Phototransduction and the Development of Early Diabetic Retinopathy.

Authors - Haitao Liu
mouseIF

J Neuroinflammation

Deficiency of TREK-1 potassium channel exacerbates blood-brain barrier damage and neuroinflammation after intracerebral hemorrhage in mice.

Authors - Yongkang Fang