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ICAM-1 Antibody 30 Publications

Rabbit Polyclonal| Catalog number: 10831-1-AP

Featured Product KD/KO validated

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Con: 51 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

ICAM-1 Antibody WB Raji cells 10831-1-AP

WB result of ICAM1 antibody (10831-1-AP, 1:1000) with si-Control and si-ICAM1 transfected Raji cells.

ICAM-1 Antibody WB HUVEC cells 10831-1-AP

HUVEC cells were subjected to SDS PAGE followed by western blot with 10831-1-AP (ICAM-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ICAM-1 Antibody IHC human lung cancer tissue 10831-1-AP

Immunohistochemical of paraffin-embedded human lung cancer using 10831-1-AP(ICAM-1 antibody) at dilution of 1:50 (under 25x lens)

ICAM-1 Antibody IF human lung cancer tissue 10831-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed human lung cancer tissue using 10831-1-AP (ICAM-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ICAM-1 Antibody IP Raji cells 10831-1-AP

IP Result of anti-ICAM-1 (IP:10831-1-AP, 4ug; Detection:10831-1-AP 1:1000) with Raji cells lysate 1320ug.

ICAM-1 Antibody IHC human liver cancer tissue 10831-1-AP

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 10831-1-AP( ICAM-1 Antibody) at dilution of 1:50 (under 40x lens)

ICAM-1 Antibody IHC human tonsillitis tissue 10831-1-AP

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 10831-1-AP( ICAM-1 Antibody) at dilution of 1:50 (under 40x lens)

ICAM-1 Antibody FC Raji cells 10831-1-AP

1X10^6 Raji cells were stained with 0.2ug ICAM-1 antibody (10831-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, bovine
Positive WB detected in:
HUVEC cells, Raji cells, L02 cells
Positive IP detected in:
Raji cells
Positive IHC detected in:
human lung cancer tissue, human liver cancer tissue, human tonsillitis tissue
Positive IF detected in:
human lung cancer tissue
Positive FC detected in:
Raji cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ICAM-1 fusion protein Ag1188
Full name:
intercellular adhesion molecule 1
Calculated molecular weight:
90 kDa
Observed molecular weight:
85-95 kDa
GenBank accession number:
BC015969
Gene ID (NCBI):
3383
Gene symbol
ICAM1
Synonyms
BB2, CD54, ICAM 1, ICAM1, P3.58

Background

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).


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