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- KD/KO Validated
IFITM2 Polyclonal antibody
IFITM2 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IP, IHC, IF, FC,ELISA
Conjugate
Unconjugated
Cat no : 12769-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 10 publications below |
WB | See 24 publications below |
IF | See 4 publications below |
FC | See 4 publications below |
Product Information
12769-1-AP targets IFITM2 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | feline, human, mouse, swine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IFITM2 fusion protein Ag3451 |
Full Name | interferon induced transmembrane protein 2 (1-8D) |
Calculated molecular weight | 132 aa, 15 kDa |
Observed molecular weight | 15 kDa |
GenBank accession number | BC009696 |
Gene symbol | IFITM2 |
Gene ID (NCBI) | 10581 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. Catalog#12769-1-AP is a rabbit polyclonal antibody produced with full-length of human IFITM2.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for IFITM2 antibody 12769-1-AP | Download protocol |
IHC protocol for IFITM2 antibody 12769-1-AP | Download protocol |
IF protocol for IFITM2 antibody 12769-1-AP | Download protocol |
IP protocol for IFITM2 antibody 12769-1-AP | Download protocol |
FC protocol for IFITM2 antibody 12769-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Hepatology IFITMs mediate viral evasion in acute and chronic hepatitis C virus infection. | ||
J Infect Dis Interferon-Induced Transmembrane Protein-Mediated Inhibition of Host Cell Entry of Ebolaviruses. | ||
Anim Cells Syst (Seoul) IFITM proteins inhibit the late step of feline foamy virus replication. | ||
PLoS One Role of rhesus macaque IFITM3(2) in simian immunodeficiency virus infection of macaques. | ||