|Positive WB detected in||MCF-7 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
12769-1-AP targets IFITM2 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||feline, human, mouse, swine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFITM2 fusion protein Ag3451|
|Full Name||interferon induced transmembrane protein 2 (1-8D)|
|Calculated molecular weight||132 aa, 15 kDa|
|Observed molecular weight||15 kDa|
|GenBank accession number||BC009696|
|Gene ID (NCBI)||10581|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. Catalog#12769-1-AP is a rabbit polyclonal antibody produced with full-length of human IFITM2.
|Product Specific Protocols|
|WB protocol for IFITM2 antibody 12769-1-AP||Download protocol|
|IHC protocol for IFITM2 antibody 12769-1-AP||Download protocol|
|IF protocol for IFITM2 antibody 12769-1-AP||Download protocol|
|IP protocol for IFITM2 antibody 12769-1-AP||Download protocol|
|FC protocol for IFITM2 antibody 12769-1-AP||Download protocol|
|Click here to view our Standard Protocols|
IFITMs mediate viral evasion in acute and chronic hepatitis C virus infection.
Opposing activities of IFITM proteins in SARS-CoV-2 infection.
J Infect Dis
Interferon-Induced Transmembrane Protein-Mediated Inhibition of Host Cell Entry of Ebolaviruses.
Anim Cells Syst (Seoul)
IFITM proteins inhibit the late step of feline foamy virus replication.
Role of rhesus macaque IFITM3(2) in simian immunodeficiency virus infection of macaques.
Host antiviral protein IFITM2 restricts pseudorabies virus replication.