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IFITM2 Antibody 23 Publications

Rabbit Polyclonal| Catalog number: 12769-1-AP

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Con: 20 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

IFITM2 Antibody IHC human breast cancer tissue 12769-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer slide using 12769-1-AP( IFITM2 Antibody) at dilution of 1:50

IFITM2 Antibody WB MCF-7 cells 12769-1-AP

MCF-7 cells were subjected to SDS PAGE followed by western blot with 12769-1-AP( IFITM2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

IFITM2 Antibody IP HepG2 cells 12769-1-AP

IP Result of anti-IFITM2 (IP:12769-1-AP, 3ug; Detection:12769-1-AP 1:300) with HepG2 cells lysate 1720ug.

IFITM2 Antibody IF HepG2 cells 12769-1-AP

Immunofluorescent analysis of HepG2 cells using 12769-1-AP(IFITM2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG

Species specificity:
human, mouse, virus
Positive WB detected in:
MCF-7 cells, human lung tissue
Positive IP detected in:
HepG2 cells
Positive IHC detected in:
human breast cancer tissue, human ovary tumor tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
IFITM2 fusion protein Ag3451
Full name:
interferon induced transmembrane protein 2 (1-8D)
Calculated molecular weight:
132aa,15 kDa
Observed molecular weight:
15 kDa
GenBank accession number:
BC009696
Gene ID (NCBI):
10581
Gene symbol
IFITM2
Synonyms
1 8D, IFITM2

Background

IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. Catalog#12769-1-AP is a rabbit polyclonal antibody produced with full-length of human IFITM2.


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