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IFITM2 Polyclonal antibody

IFITM2 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 12769-1-AP

Synonyms

1 8D, IFITM2



Tested Applications

Positive WB detected inMCF-7 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12769-1-AP targets IFITM2 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFITM2 fusion protein Ag3451
Full Name interferon induced transmembrane protein 2 (1-8D)
Calculated molecular weight 132 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC009696
Gene symbol IFITM2
Gene ID (NCBI) 10581
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. Catalog#12769-1-AP is a rabbit polyclonal antibody produced with full-length of human IFITM2.

Protocols

Product Specific Protocols
WB protocol for IFITM2 antibody 12769-1-APDownload protocol
IHC protocol for IFITM2 antibody 12769-1-APDownload protocol
IF protocol for IFITM2 antibody 12769-1-APDownload protocol
IP protocol for IFITM2 antibody 12769-1-APDownload protocol
FC protocol for IFITM2 antibody 12769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Hepatology

IFITMs mediate viral evasion in acute and chronic hepatitis C virus infection.

Authors - Florian Wrensch
humanFC

bioRxiv

Opposing activities of IFITM proteins in SARS-CoV-2 infection.

Authors - Guoli Shi
humanWB

J Infect Dis

Interferon-Induced Transmembrane Protein-Mediated Inhibition of Host Cell Entry of Ebolaviruses.

Authors - Florian Wrensch
humanWB

Anim Cells Syst (Seoul)

IFITM proteins inhibit the late step of feline foamy virus replication.

Authors - Jinsun Kim
humanWB

PLoS One

Role of rhesus macaque IFITM3(2) in simian immunodeficiency virus infection of macaques.

Authors - Michael Winkler
pigWB

Virus Res

Host antiviral protein IFITM2 restricts pseudorabies virus replication.

Authors - Jingying Xie