IFN Gamma Polyclonal antibody
IFN Gamma Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 15365-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Recombinant protein protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 18 publications below |
| IHC | See 14 publications below |
| IF | See 6 publications below |
Product Information
15365-1-AP targets IFN Gamma in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IFN Gamma fusion protein Ag8321 |
| Full Name | IFN gamma |
| Calculated molecular weight | 166 aa, 19 kDa |
| GenBank accession number | BC070256 |
| Gene symbol | IFNG |
| Gene ID (NCBI) | 3458 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IFN gamma (IFNG) is a soluble cytokine that is the only member of the type II class of IFN. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IFN Gamma antibody 15365-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Physiol Dehydroepiandrosterone stimulates inflammation and impairs ovarian functions of polycystic ovary syndrome. | ||
Am J Chin Med Total Glycosides of Peony Protects Against Inflammatory Bowel Disease by Regulating IL-23/IL-17 Axis and Th17/Treg Balance. | ||
Cancer Res Low-dose IFN-γ induces tumor cell stemness in the tumor microenvironment of non-small cell lung cancer. | ||
Mol Genet Genomics A comparison of CRISPR/Cas9 and siRNA-mediated ALDH2 gene silencing in human cell lines. | ||
Transplantation Decreased percentages of regulatory T cells are necessary to activate Th1-th17-th22 responses during acute rejection of the peripheral nerve xenotransplantation in mice. | ||
J Huazhong Univ Sci Technolog Med Sci Implications of Th1 and Th17 cells in pathogenesis of oral lichen planus. |
