IGFBP1 Polyclonal antibody

IGFBP1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13981-1-AP

Synonyms

AFBP, hIGFBP 1, IBP 1, IBP1, IGF binding protein 1, IGF BP25, IGFBP 1, IGFBP1, Placental protein 12, PP12



Tested Applications

Positive WB detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

13981-1-AP targets IGFBP1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGFBP1 fusion protein Ag5026
Full Name IGF binding protein 1
Calculated molecular weight 28 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC057806
Gene symbol IGFBP1
Gene ID (NCBI) 3484
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGF-binding proteins (IGFBPs) family contains members like IGFBP-1, -2, -3, -4,-5 and-6. IGFBPs prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. IGF-I physically interacts with IGFBP-1 and that IGFBP-1 also binds to an integrin receptor, but the effect of IGFBP-1 on cell migration may be independent of IGF-I and is probably mediated through the alpha 5 beta 1 integrin. Transgenic mice models demonstrated that IGFBPs played important roles in the pathogenesis of obesity and INS resistance. Studies conducted in humans demonstrated the close relation between IGFBPs and the components of the metabolic syndrome.

Protocols

Product Specific Protocols
WB protocol for IGFBP1 antibody 13981-1-APDownload protocol
IHC protocol for IGFBP1 antibody 13981-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Med Rep

PMID: 25310700

Authors - Tao Feng
humanWB

Prenat Diagn

Differences in IGF axis-related proteins in amniotic fluid of trisomy 21 and trisomy 18 using a multiple reaction monitoring (MRM) approach.

Authors - Guangrui Lai
humanWB

Reprod Biol Endocrinol

PMID: 29925391

Authors - Cheng Li
humanWB

Biol Reprod

IGFBP1 Involved in the Decreased Birth Weight Due to Fetal High Estrogen Exposure in Mice.

Authors - Min Jin