IGFBP3 Polyclonal antibody

IGFBP3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10189-2-AP

Synonyms

BP 53, IBP 3, IBP3, IGF binding protein 3, IGFBP 3, IGFBP3



Tested Applications

Positive WB detected inmouse colon tissue, HeLa cells, A2780 cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10189-2-AP targets IGFBP3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity chicken, human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGFBP3 fusion protein Ag0242
Full Name insulin-like growth factor binding protein 3
Calculated molecular weight 32 kDa
Observed molecular weight 32-37 kDa
GenBank accession numberBC000013
Gene symbol IGFBP3
Gene ID (NCBI) 3486
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid.

Protocols

Product Specific Protocols
WB protocol for IGFBP3 antibody 10189-2-APDownload protocol
IHC protocol for IGFBP3 antibody 10189-2-APDownload protocol
IF protocol for IGFBP3 antibody 10189-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Environ Toxicol Pharmacol

Protective potential of epigallocatechin-3-gallate against benign prostatic hyperplasia in metabolic syndrome rats.

Authors - Jinglou Chen
humanWB

Stem Cell Res

Normoxia is not favorable for maintaining stemness of human endothelial progenitor cells.

Authors - Yiqiu Lin
chickenWB

Cell Signal

PMID: 28734787

Authors - Jie Yang
humanWB

Front Pharmacol

Quercetin Attenuates Atherosclerosis via Modulating Oxidized LDL-Induced Endothelial Cellular Senescence.

Authors - Yue-Hua Jiang
humanWB

Clin Epigenetics

Silencing HOXD10 by promoter region hypermethylation activates ERK signaling in hepatocellular carcinoma.

Authors - Yulin Guo
ratIHC

Cell Prolif

Exosomes derived from miR-375-overexpressing human adipose mesenchymal stem cells promote bone regeneration.

Authors - Si Chen