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IGFBP3 Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 10189-2-AP

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Con: 37 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

IGFBP3 Antibody WB mouse colon tissue 10189-2-AP

mouse colon tissue were subjected to SDS PAGE followed by western blot with 10189-2-AP (IGFBP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

IGFBP3 Antibody IHC human breast cancer tissue 10189-2-AP

Immunohistochemical of paraffin-embedded human breast cancer using 10189-2-AP(IGFBP3 antibody) at dilution of 1:50 (under 10x lens)

IGFBP3 Antibody IF HeLa cells 10189-2-AP

Immunofluorescent analysis of HeLa cells using 10189-2-AP(IGFBP3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, chicken, rat
Positive WB detected in:
mouse colon tissue, HeLa cells, A2780 cells, HepG2 cells
Positive IHC detected in:
human breast cancer tissue, human colon cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:200-1:1000
IHC : 1:20-1:200
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
IGFBP3 fusion protein Ag0242
Full name:
insulin-like growth factor binding protein 3
Calculated molecular weight:
32 kDa
Observed molecular weight:
32 kDa
GenBank accession number:
BC000013
Gene ID (NCBI):
3486
Gene symbol
IGFBP3
Synonyms
BP 53, IBP 3, IBP3, IGF binding protein 3, IGFBP 3, IGFBP3

Background

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid.


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