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- KD/KO Validated
IGFBP3 Polyclonal antibody
IGFBP3 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10189-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse colon tissue, HeLa cells, A2780 cells, HepG2 cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
Product Information
10189-2-AP targets IGFBP3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | chicken, human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IGFBP3 fusion protein Ag0242 |
| Full Name | insulin-like growth factor binding protein 3 |
| Calculated molecular weight | 32 kDa |
| Observed molecular weight | 32-37 kDa |
| GenBank accession number | BC000013 |
| Gene symbol | IGFBP3 |
| Gene ID (NCBI) | 3486 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IGFBP3 antibody 10189-2-AP | Download protocol |
| IHC protocol for IGFBP3 antibody 10189-2-AP | Download protocol |
| IF protocol for IGFBP3 antibody 10189-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Environ Toxicol Pharmacol Protective potential of epigallocatechin-3-gallate against benign prostatic hyperplasia in metabolic syndrome rats. | ||
Stem Cell Res Normoxia is not favorable for maintaining stemness of human endothelial progenitor cells. | ||
Front Pharmacol Quercetin Attenuates Atherosclerosis via Modulating Oxidized LDL-Induced Endothelial Cellular Senescence. | ||
Clin Epigenetics Silencing HOXD10 by promoter region hypermethylation activates ERK signaling in hepatocellular carcinoma. | ||
Cell Prolif Exosomes derived from miR-375-overexpressing human adipose mesenchymal stem cells promote bone regeneration. |
