IGFBP4 Polyclonal antibody

IGFBP4 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18500-1-AP

Synonyms

BP 4, HT29 IGFBP, IBP 4, IBP4, IGF binding protein 4, IGFBP 4, IGFBP4



Tested Applications

Positive WB detected inC6 cells
Positive IHC detected inhuman liver tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 18500-1-AP is IGFBP4 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Cited Reactivity chicken, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGFBP4 fusion protein Ag13347
Full Name insulin-like growth factor binding protein 4
Calculated molecular weight 28 kDa
Observed molecular weight 28-40 kDa
GenBank accession numberBC016041
Gene symbol IGFBP4
Gene ID (NCBI) 3487
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGFBP4, also named as IBP4, is a 258 amino acid protein, which contains one IGFBP N-terminal domain and one thyroglobulin type-1 domain. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. IGFBP4 is a secreted protein and calculated molecular weight is 28 kDa.

Protocols

Product Specific Protocols
WB protocol for IGFBP4 antibody 18500-1-APDownload protocol
IHC protocol for IGFBP4 antibody 18500-1-APDownload protocol
IF protocol for IGFBP4 antibody 18500-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
chickenWB

Cell Signal

PMID: 28734787

Authors - Jie Yang
humanWB

Theranostics

Proteomic analysis of intracellular protein corona of nanoparticles elucidates nano-trafficking network and nano-bio interactions.

Authors - Mengmeng Qin