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IKBKB Antibody 13 Publications

Rabbit Polyclonal| Catalog number: 15649-1-AP

Featured Product KD/KO validated

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Con: 20 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

IKBKB Antibody WB Jurkat cells 15649-1-AP

WB result of IKBKB antibody (15649-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-IKBKB transfected Jurkat cells

IKBKB Antibody WB Jurkat cells 15649-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 15649-1-AP (IKBKB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

IKBKB Antibody IHC human liver cancer tissue 15649-1-AP

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 15649-1-AP (IKBKB antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IKBKB Antibody IHC human liver cancer tissue 15649-1-AP

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 15649-1-AP (IKBKB antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat
Positive WB detected in:
Jurkat cells, K-562 cells, HepG2 cells
Positive IHC detected in:
human liver cancer tissue, human prostate cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:200-1:1000
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
IKBKB fusion protein Ag8191
Full name:
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
Calculated molecular weight:
756aa,81 kDa;256aa,29 kDa
Observed molecular weight:
80-87 kDa
GenBank accession number:
BC006231
Gene ID (NCBI):
3551
Gene symbol
IKBKB
Synonyms
I kappa B kinase 2, I kappa B kinase beta, IKBKB, IKK B, IKK beta, IKK2, IKKB, IκB, NFKBIKB

Background

IKBKB, also named as IKKB, IKK2, NFKBIKB and IKK-B, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and I-kappa-B kinase subfamily. IKBKB is a Serine kinase that plays an essential role in the NF-kappa-B signaling pathway. It acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. In addition to the NF-kappa-B inhibitors, IKBKB phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. It also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, IKBKB acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.


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