IL-15RA Polyclonal antibody

IL-15RA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16744-1-AP

Synonyms

IL 15 receptor subunit alpha, IL 15R alpha, IL 15RA, IL15RA, IL-15RA, interleukin 15 receptor, alpha, sIL 15 receptor subunit alpha, sIL 15R alpha, sIL 15RA



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, human spleen tissue, human kidney tissue, human heart tissue, human testis tissue, human skin tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16744-1-AP targets IL-15RA in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-15RA fusion protein Ag10151
Full Name interleukin 15 receptor, alpha
Calculated Molecular Weight 231 aa, 24 kDa
Observed Molecular Weight 55-65 kDa
GenBank Accession NumberBC107777
Gene Symbol IL-15RA
Gene ID (NCBI) 3601
RRIDAB_2233644
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-15 (IL-15) is a pleiotropic cytokine that plays an important role in both innate and adaptive immunity. IL-15 is mainly produced by activated monocytes, macrophages and dendritic cells, and is structurally similar to IL-2 (PMID: 8178155; 12401478). These two cytokines share the same IL-2/15Rβ and common γ-chain receptor subunits. In addition, IL-2 and IL-15 have their own private α-chain receptor subunit, IL-2Rα and IL-15Rα, respectively (PMID: 7641685). The IL-15Rα chain is expressed by many cell types including monocytes, DCs, NK, T cells and fibroblasts. Several isoforms of IL-15Rα exist and are generated either by alternative splicing or by proteolytic cleavage (PMID: 10480910; 15265897). The calculated molecular weight of full-length IL-15Rα is 28 kDa, IL-15Rα can be glycosylated, and larger apparent molecular weights of 55-65 kDa have been reported by some researches (PMID: 10480910; 15976182; 15671076; 17912462).

Protocols

Product Specific Protocols
WB protocol for IL-15RA antibody 16744-1-APDownload protocol
IHC protocol for IL-15RA antibody 16744-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nat Commun

An Artificial Intelligence-guided signature reveals the shared host immune response in MIS-C and Kawasaki disease.

Authors - Pradipta Ghosh
humanWB

Clin Sci (Lond)

HMGB1-induced autophagy facilitates hepatic stellate cells activation: a new pathway in liver fibrosis.

Authors - Jing Li
humanIHC

Stem Cells Dev

Human gastric cancer mesenchymal stem cell-derived IL15 contributes to tumor cell EMT via up-regulation of Tregs ratio and PD-1 expression in CD4+T cell.

Authors - Li Sun
humanIHC

Immunology

Interleukin-15 receptor α expression in inflammatory bowel disease patients before and after normalization of inflammation with infliximab.

Authors - Perrier Clémentine C
human,hamsterIHC

bioRxiv

AI-guided discovery of the invariant host response to viral pandemics.

Authors - Debashis Sahoo
humanIHC

EBioMedicine

AI-guided discovery of the invariant host response to viral pandemics

Authors - Debashis Sahoo