|Positive WB detected in||mouse liver tissue, mouse lung tissue, Jurkat cells, human placenta tissue|
|Positive IF detected in||RAW 264.7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 16765-1-AP is IL-1 Alpha Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IL-1 Alpha fusion protein Ag10339|
|Full Name||interleukin 1, alpha|
|Calculated molecular weight||271 aa, 31 kDa|
|Observed molecular weight||30 kDa, 17 kDa|
|GenBank accession number||BC013142|
|Gene ID (NCBI)||3552|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Interleukin-1α (IL-1α) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1αis a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1αalso has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. IL-1αfunctions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1αwas found to affect the cell cycle of osteosarcoma cell to reduce cell growth. IL1-alpha is initially translated as approximately 30-kD polypeptides that are processed to approximately 17.5-kD polypeptides prior to, or during, release from macrophages.
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