IL-1 Alpha Polyclonal antibody
IL-1 Alpha Polyclonal Antibody for IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 16765-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse liver tissue, mouse lung tissue, Jurkat cells, human placenta tissue |
Positive IF detected in | RAW 264.7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 4 publications below |
IHC | See 4 publications below |
Product Information
The immunogen of 16765-1-AP is IL-1 Alpha Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IL-1 Alpha fusion protein Ag10339 |
Full Name | interleukin 1, alpha |
Calculated molecular weight | 271 aa, 31 kDa |
Observed molecular weight | 30 kDa, 17 kDa |
GenBank accession number | BC013142 |
Gene symbol | IL1A |
Gene ID (NCBI) | 3552 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interleukin-1α (IL-1α) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1αis a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1αalso has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. IL-1αfunctions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1αwas found to affect the cell cycle of osteosarcoma cell to reduce cell growth. IL1-alpha is initially translated as approximately 30-kD polypeptides that are processed to approximately 17.5-kD polypeptides prior to, or during, release from macrophages.
Protocols
Product Specific Protocols | |
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WB protocol for IL-1 Alpha antibody 16765-1-AP | Download protocol |
IF protocol for IL-1 Alpha antibody 16765-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Naunyn Schmiedebergs Arch Pharmacol Crocin attenuates isoprenaline-induced myocardial fibrosis by targeting TLR4/NF-κB signaling: connecting oxidative stress, inflammation, and apoptosis. | ||
Mol Med Rep Ameliorative effects and mechanism of crocetin in arsenic trioxide‑induced cardiotoxicity in rats. | ||
J Tissue Viability Non-invasive detection of local tissue responses to predict pressure ulcer development in mouse models. | ||
Front Pharmacol Effects of Ketamine on Levels of Inflammatory Cytokines IL-6, IL-1β, and TNF-α in the Hippocampus of Mice Following Acute or Chronic Administration. |