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Inhibin beta A Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 10651-1-AP

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Con: 20 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

Inhibin beta A Antibody WB A549 cells 10651-1-AP

A549 cells were subjected to SDS PAGE followed by western blot with 10651-1-AP(Inhibin beta A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Inhibin beta A Antibody IHC human lung cancer tissue 10651-1-AP

Immunohistochemical of paraffin-embedded human lung cancer using 10651-1-AP(Inhibin beta A antibody) at dilution of 1:50 (under 10x lens)

Inhibin beta A Antibody IHC human lung cancer tissue 10651-1-AP

Immunohistochemical of paraffin-embedded human lung cancer using 10651-1-AP(Inhibin beta A antibody) at dilution of 1:50 (under 40x lens)

Inhibin beta A Antibody IF A549 cells 10651-1-AP

Immunofluorescent analysis of A549 cells using 10651-1-AP(Inhibin beta A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse
Positive WB detected in:
A549 cells, BGC-823 cells, HeLa cells, HepG2 cells, MCF-7 cells, SGC-7901 cells
Positive IHC detected in:
human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
A549 cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:200
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Inhibin beta A fusion protein Ag1001
Full name:
inhibin, beta A
Calculated molecular weight:
47 kDa
Observed molecular weight:
47 kDa
GenBank accession number:
BC007858
Gene ID (NCBI):
3624
Gene symbol
INHBA
Synonyms
Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, Inhibin β-A, inhibin, beta A

Background

Inhibin is a 32-kD dimeric glycoprotein consisting of  an alpha subunit and one of two beta subunits (beta A or beta B) leading to inhibin A or B, respectively, which was isolated and cloned on the basis of its ability to inhibit FSH release from the pituitary. Inhibin βA forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin βA can also bind the βB and α isoforms to form activin AB and inhibin, respectively.


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