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IRAK1 Antibody

IRAK1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10478-2-AP

Synonyms

IRAK, IRAK 1, IRAK1, pelle



Tested Applications

Positive WB detected inA549 cells, PC-13 cells, PC-12 cells, RAW 264.7 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10478-2-AP targets IRAK1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human, mouse, rat, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRAK1 fusion protein Ag0728
Full Name interleukin-1 receptor-associated kinase 1
Calculated molecular weight 77 kDa
Observed molecular weight 68-80 kDa
GenBank accession number BC014963
Gene symbol IRAK1
Gene ID (NCBI) 3654
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-1 receptor-associated kinases (IRAKs) are a unique family of death domain containing protein kinases that play a key role in initiating innate immune response against foreign pathogens. They are involved in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. IRAK1 is the first member of this kinase family. Upon ligand binding to TLR/IL-1R, IRAK1 is recruited by MYD88 to the receptor-signaling complex, the association leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Hyper-phosphorylated IRAK1 then disengages from the receptor complex, and forms a cytosolic IRAK1-TRAF6 complex. TRAF6 then interacts with TAK and TAB, resulting in eventual activation of the NF-κB and MAPK pathways. Phosphorylated IRAK1 also undergoes ubiquitin-mediated degradation or sumoylation, which results in nuclear translocation and transcriptional activation of inflammatory target genes. (PMID: 17890055; 12620219)

Protocols

Product Specific Protocols
WB protocol for IRAK1 antibody 10478-2-APDownload protocol
IHC protocol for IRAK1 antibody 10478-2-APDownload protocol
IF protocol for IRAK1 antibody 10478-2-APDownload protocol
IP protocol for IRAK1 antibody 10478-2-APDownload protocol
FC protocol for IRAK1 antibody 10478-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell Proteomics

Quantification of dynamic protein interactions and phosphorylation in LPS signaling pathway by SWATH-MS.

Authors - Xiurong Wu
human,mouseWB,IHC

Artif Cells Nanomed Biotechnol

Targeted delivery of microRNA 146b mimic to hepatocytes by lactosylated PDMAEMA nanoparticles for the treatment of NAFLD.

Authors - Shuying He
mouseWB

Int Immunopharmacol

Dioscin reduces lipopolysaccharide-induced inflammatory liver injury via regulating TLR4/MyD88 signal pathway.

Authors - Hong Yao
ratWB

J Ethnopharmacol

Influence of the Total Saponin Fraction from Dioscorea nipponica Makino on TLR2/4-IL1R Receptor Singnal Pathway in Rats of Gouty Arthritis.

Authors - Qi Zhou
ratWB

Molecules

Sal B Alleviates Myocardial Ischemic Injury by Inhibiting TLR4 and the Priming Phase of NLRP3 Inflammasome.

Authors - Yang Hu
sheepWB

Mediators Inflamm

Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells.

Authors - Zhongjia Jiang