IRAK1 Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 10478-2-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 34 μg/150 μl

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Species specificity:
human, mouse, rat, sheep

Positive WB detected in:
A549 cells,HeLa cells, A549 cells, HEK-293 cells, Jurkat cells, K-562 cells, MCF-7 cells, PC-13 cells, PC-12 cells

Positive IP detected in:
HeLa cells

Positive IHC detected in:
human breast cancer tissue, human breast cancer tissue, human lung cancer tissue

Positive IF detected in:
A549 cells

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:200
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
interleukin-1 receptor-associated kinase 1

Calculated molecular weight:
77 kDa

Observed molecular weight:
68-80 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
IRAK1

Synonyms
IRAK, IRAK 1, IRAK1, pelle
Background

Interleukin-1 receptor-associated kinases (IRAKs) are a unique family of death domain containing protein kinases that play a key role in initiating innate immune response against foreign pathogens. They are involved in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. IRAK1 is the first member of this kinase family. Upon ligand binding to TLR/IL-1R, IRAK1 is recruited by MYD88 to the receptor-signaling complex, the association leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Hyper-phosphorylated IRAK1 then disengages from the receptor complex, and forms a cytosolic IRAK1-TRAF6 complex. TRAF6 then interacts with TAK and TAB, resulting in eventual activation of the NF-κB and MAPK pathways. Phosphorylated IRAK1 also undergoes ubiquitin-mediated degradation or sumoylation, which results in nuclear translocation and transcriptional activation of inflammatory target genes. (PMID: 17890055; 12620219)


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