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  • Featured Product
  • KD/KO Validated

MUM1/IRF4 Polyclonal antibody

MUM1/IRF4 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 11247-2-AP

Synonyms

IRF 4, IRF4, LSIRF, Multiple myeloma oncogene 1, MUM1, MUM1/IRF4, NF EM5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MUM1/IRF4 Polyclonal antibody (11247-2-AP)

    WB analysis using 11247-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using MUM1/IRF4 Polyclonal antibody (11247-2-AP)

    WB analysis of mouse spleen using 11247-2-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW264.7 using MUM1/IRF4 Polyclonal antibody (11247-2-AP)

    WB analysis of RAW264.7 using 11247-2-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MUM1/IRF4 Polyclonal antibody (11247-2-AP)

    WB analysis of Jurkat using 11247-2-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using MUM1/IRF4 Polyclonal antibody (11247-2-AP)

    WB analysis of mouse thymus using 11247-2-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using MUM1/IRF4 Polyclonal antibody (11247-2-AP)

    WB analysis of mouse lung using 11247-2-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using MUM1/IRF4 Polyclonal antibody (11247-2-AP)

    WB analysis of Raji using 11247-2-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse spleen tissue using MUM1/IRF4 Polyclonal antibody (11247-2-AP)

    IP experiment of mouse spleen using 11247-2-AP

    IP result of anti-MUM1/IRF4 (IP:11247-2-AP, 3ug; Detection:11247-2-AP 1:500) with mouse spleen tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human thymus tissue using MUM1/IRF4 Polyclonal antibody (11247-2-AP)

    IHC staining of human thymus using 11247-2-AP

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thymus tissue using MUM1/IRF4 Polyclonal antibody (11247-2-AP)

    IHC staining of human thymus using 11247-2-AP

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MUM1/IRF4 Polyclonal antibody (11247-2-AP)

    IHC staining of human tonsillitis using 11247-2-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MUM1/IRF4 Polyclonal antibody (11247-2-AP)

    IHC staining of human tonsillitis using 11247-2-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using MUM1/IRF4 Polyclonal antibody (11247-2-AP)

    IHC staining of human lymphoma using 11247-2-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using MUM1/IRF4 Polyclonal antibody (11247-2-AP)

    IHC staining of human lymphoma using 11247-2-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of Ramos cells using human MUM1/IRF4 Polyclonal antibody (11247-2-AP)

    FC experiment of Ramos using 11247-2-AP

    1X10^6 Ramos cells were intracellularly stained with 0.5 ug Anti-Human MUM1/IRF4 (11247-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of Ramos cells using human MUM1/IRF4 Polyclonal antibody (11247-2-AP)

    FC experiment of Ramos using 11247-2-AP

    (blue)1x10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human MUM1/IRF4 (11247-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) at dilution 1:1000(red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inmouse spleen tissue, Jurkat cells, mouse thymus tissue, Raji cells, RAW264.7, mouse lung tissue, rat spleen tissue
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman thymus tissue, human lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRamos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11247-2-AP targets MUM1/IRF4 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUM1/IRF4 fusion protein Ag1762
Full Name IRF 4
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC015752
Gene Symbol IRF4
Gene ID (NCBI) 3662
RRIDAB_2264940
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRF4 is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the ISRE of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

Protocols

Product Specific Protocols
WB protocol for MUM1/IRF4 antibody 11247-2-APDownload protocol
IHC protocol for MUM1/IRF4 antibody 11247-2-APDownload protocol
IP protocol for MUM1/IRF4 antibody 11247-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Diabetes

Obese Skeletal Muscle-Expressed Interferon Regulatory Factor 4 Transcriptionally Regulates Mitochondrial Branched-Chain Aminotransferase Reprogramming Metabolome.

Authors - Ting Yao
  • KD Validated
View Article
mouseWB

Nanotoxicology

Macrophage polarization and activation at the interface of multi-walled carbon nanotube-induced pulmonary inflammation and fibrosis.

Authors - Jie Dong
View Article
humanIHC

Diabetes Res Clin Pract

fMaggot treatment promotes healing of diabetic foot ulcer wounds possibly by upregulating Treg levels.

Authors - Jie Zhang
View Article
mouseIHC

Int Immunopharmacol

IRF-4 deficiency reduces inflammation and kidney fibrosis after folic acid-induced acute kidney injury.

Authors - Meixin Chen
  • KO Validated
View Article
mouseWB,IHC

Mediators Inflamm

MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression.

Authors - Aijun Jia
View Article
mouseWB

Adv Sci (Weinh)

IRF4 in Skeletal Muscle Regulates Exercise Capacity via PTG/Glycogen Pathway.

Authors - Xiaopeng Zhu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Shunbin (Verified Customer) (05-01-2023)

One of the best antibody we have used for western blotting. 1:10,000 produces a single specific band. original concentration: 440 ug/ml

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10,000
  • Cell Tissue Type: B/T cell lines
MUM1/IRF4 Antibody Western Blot validation (1:10,000 dilution) in B/T cell lines (Cat no:11247-2-AP)