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ISG15 Antibody 12 Publications

Rabbit Polyclonal| Catalog number: 15981-1-AP

Featured Product KD/KO validated

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Con: 32 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

ISG15 Antibody WB human brain tissue 15981-1-AP

human brain tissue were subjected to SDS PAGE followed by western blot with 15981-1-AP(ISG15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ISG15 Antibody IHC human lung cancer tissue 15981-1-AP

Immunohistochemical of paraffin-embedded human lung cancer using 15981-1-AP(ISG15 antibody) at dilution of 1:50 (under 40x lens)

ISG15 Antibody IP HeLa cells 15981-1-AP

IP Result of anti-ISG15 (IP:15981-1-AP, 4ug; Detection:15981-1-AP 1:500) with HeLa cells lysate 3200ug.

ISG15 Antibody IHC human pancreas cancer tissue 15981-1-AP

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15981-1-AP( ISG15 Antibody) at dilution of 1:50 (under 40x lens)

ISG15 Antibody IF HeLa cells 15981-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 15981-1-AP(ISG15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, goat
Positive WB detected in:
human brain tissue
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human lung cancer tissue, human pancreas cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ISG15 fusion protein Ag8782
Full name:
ISG15 ubiquitin-like modifier
Calculated molecular weight:
165aa,18 kDa
Observed molecular weight:
15-17 kDa
GenBank accession number:
BC009507
Gene ID (NCBI):
9636
Gene symbol
ISG15
Synonyms
G1P2, hUCRP, IFI15, IP17, ISG15, ISG15 ubiquitin like modifier, Ubiquitin like protein ISG15, UCRP

Background

ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA) and -beta (IFNB). ISG15 forms covalent conjugates with its target proteins in a process called ISGylation, which in mammals is known to play a role in antiviral immunity. ISG15 proteins possess two ubiquitin-like (UBL) domains and a highly conserved C-terminal LRGG sequence, the latter being known as the ubiquitin conjugation motif. Intracellular ISG15 are conjugated, via the LRGG motif, to target proteins through a process called ISGylation, which resembles largely ubiquitination, the process of formation of ubiquitin conjugates. Unconjugated extracellular ISG15, which are released from several types of human and murine cells, are known to possess cytokine-like activity.


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