ISG15 Antibody 6 Publications

Rabbit Polyclonal| Catalog number: 15981-1-AP

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Freight/Packing

Con: 32 μg/150 μl

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Species specificity:
human, goat

Positive WB detected in:
human brain tissue

Positive IP detected in:
HeLa cells

Positive IHC detected in:
human lung cancer tissue, human pancreas cancer tissue

Positive IF detected in:
HeLa cells

Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
ISG15 ubiquitin-like modifier

Calculated molecular weight:
165aa,18 kDa

Observed molecular weight:
15-17 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
ISG15

Synonyms
G1P2, hUCRP, IFI15, IP17, ISG15, ISG15 ubiquitin like modifier, Ubiquitin like protein ISG15, UCRP
Background

ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA) and -beta (IFNB). ISG15 forms covalent conjugates with its target proteins in a process called ISGylation, which in mammals is known to play a role in antiviral immunity. ISG15 proteins possess two ubiquitin-like (UBL) domains and a highly conserved C-terminal LRGG sequence, the latter being known as the ubiquitin conjugation motif. Intracellular ISG15 are conjugated, via the LRGG motif, to target proteins through a process called ISGylation, which resembles largely ubiquitination, the process of formation of ubiquitin conjugates. Unconjugated extracellular ISG15, which are released from several types of human and murine cells, are known to possess cytokine-like activity.


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