|Positive WB detected in||mouse testis tissue, HepG2 cells|
|Positive IHC detected in||human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14142-1-AP targets ISYNA1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ISYNA1 fusion protein Ag5272|
|Full Name||inositol-3-phosphate synthase 1|
|Calculated molecular weight||61 kDa|
|Observed molecular weight||61 kDa|
|GenBank accession number||BC066902|
|Gene ID (NCBI)||51477|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Myo-inositol 3-phosphate synthase (ISYNA1/IP synthase) is a rate-limiting enzyme that catalyzes the first step in the biosynthesis of all inositol containing compounds. It converts glucose 6-phosphate to Myo-inositol 3-phosphate.(PMID:21841945). The native enzyme is typically a homotrimermade up of 68-kDa subunits in mammalian cells and a homotetramer in yeast and plant cells. There are three new isoforms of 62, 43, and 16kDa detected suggesting ISYNA1 is a completely different holoenzyme. But only the brain and testis manifest the 68-kDa isoform, whereas the pancreas has a slightly smaller isoformat 67 kDa. The intestine has a completely unique isoformprofile comprising 62,43, 20kDa isoforms(PMID:19188364).
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