ISYNA1 Polyclonal antibody

ISYNA1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF

Conjugate

Unconjugated

Cat no : 14142-1-AP

Synonyms

hINO1, hIPS, Ino1, INOS, IPS, IPS 1, ISYNA1, MI 1 P synthase, MIP synthase



Tested Applications

Positive WB detected inmouse testis tissue, HepG2 cells, rat testis tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14142-1-AP targets ISYNA1 in WB, IHC, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ISYNA1 fusion protein Ag5272
Full Name inositol-3-phosphate synthase 1
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC066902
Gene Symbol ISYNA1
Gene ID (NCBI) 51477
RRIDAB_2233801
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myo-inositol 3-phosphate synthase (ISYNA1/IP synthase) is a rate-limiting enzyme that catalyzes the first step in the biosynthesis of all inositol containing compounds. It converts glucose 6-phosphate to Myo-inositol 3-phosphate.(PMID:21841945). The native enzyme is typically a homotrimermade up of 68-kDa subunits in mammalian cells and a homotetramer in yeast and plant cells. There are three new isoforms of 62, 43, and 16kDa detected suggesting ISYNA1 is a completely different holoenzyme. But only the brain and testis manifest the 68-kDa isoform, whereas the pancreas has a slightly smaller isoformat 67 kDa. The intestine has a completely unique isoformprofile comprising 62,43, 20kDa isoforms(PMID:19188364).

Protocols

Product Specific Protocols
WB protocol for ISYNA1 antibody 14142-1-APDownload protocol
IHC protocol for ISYNA1 antibody 14142-1-APDownload protocol
IF protocol for ISYNA1 antibody 14142-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Acta Pharmacol Sin

Ginsenoside Rb1 alleviates colitis in mice via activation of endoplasmic reticulum-resident E3 ubiquitin ligase Hrd1 signaling pathway.

Authors - Jian-Yi Dong
mouseWB

Front Pharmacol

Naringin Exerts Therapeutic Effects on Mice Colitis: A Study Based on Transcriptomics Combined With Functional Experiments

Authors - Jianyi Dong
ratWB, IF

Alcohol Clin Exp Res

Ethanol supports macrophage recruitment and reinforces invasion and migration of lewis lung carcinoma.

Authors - Keke Yu
mouseWB

Mol Reprod Dev

Comparative proteomic analysis of proteins involved in oocyte meiotic maturation in mice.

Authors - Cao Senyang S