JAK1 Monoclonal antibody

JAK1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3H7A8

Cat no : 66466-1-Ig

Synonyms

JAK 1, JAK1, JAK1A, JAK1B, Janus kinase 1, JTK3, Tyrosine protein kinase JAK1



Tested Applications

Positive WB detected inJurkat cells, rat spleen tissue
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 5 publications below

Product Information

66466-1-Ig targets JAK1 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen JAK1 fusion protein Ag17940
Full Name Janus kinase 1 (a protein tyrosine kinase)
Calculated molecular weight 1154 aa, 133 kDa
Observed molecular weight 133 kDa
GenBank accession numberBC132729
Gene symbol JAK1
Gene ID (NCBI) 3716
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Janus kinase 1(JAK1), is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. JAK1 is essential for signaling for certain type I and type II cytokines. JAK1 plays a critical role in initiating responses to multiple major cytokine receptor families. Expression of JAK1 in cancer cells enables individual cells to contract, potentially allowing them to escape their tumor and metastasize to other parts of the body.

Protocols

Product Specific Protocols
WB protocol for JAK1 antibody 66466-1-IgDownload protocol
IHC protocol for JAK1 antibody 66466-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Eur J Pharmacol

Integration of transcriptomics and network analysis deciphers the mechanisms of baicalein in improving learning and memory impairment in senescence-accelerated mouse prone 8 (SAMP8).

Authors - Jiaqi Li
humanWB

J Cell Physiol

CCL21/CCR7 interaction promotes EMT and enhances the stemness of OSCC via a JAK2/STAT3 signaling pathway.

Authors - Yang Chen
humanWB

Int J Biol Sci

PMID: 29805309

Authors - Linna Xie
humanWB

Cells

CEP55 Promotes Cell Motility via JAK2⁻STAT3⁻MMPs Cascade in Hepatocellular Carcinoma.

Authors - Minjing Li
mouseWB

Oxid Med Cell Longev

Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blocking the TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response.

Authors - Guanghui Wang