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JNK Antibody 45 Publications

Rabbit Polyclonal| Catalog number: 51151-1-AP

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Con: 28 μg/150 μl

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Tested Applications

Applications:
WB, IP, IF, ELISA
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Images See all

JNK Antibody WB SH-SY5Y cells 51151-1-AP

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 51151-1-AP(JNK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

JNK Antibody IP HEK-293 cells 51151-1-AP

IP Result of anti-JNK (IP:51151-1-AP, 4ug; Detection:51151-1-AP 1:600) with HEK-293 cells lysate 2000ug.

JNK Antibody IF SH-SY5Y cells 51151-1-AP

Immunofluorescent analysis of ( -20℃ Acetone ) fixed SH-SY5Y cells using 51151-1-AP(JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, A. sphaerocephala Krasch, rat
Positive WB detected in:
SH-SY5Y cells, A431 cells, HEK-293 cells, HeLa cells, HT-1080 cells, PC-3 cells
Positive IP detected in:
HEK-293 cells
Positive IF detected in:
SH-SY5Y cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
mitogen-activated protein kinase 8
Calculated molecular weight:
48 kDa
Observed molecular weight:
44-48 kDa
GenBank accession number:
BC130570
Gene ID (NCBI):
5599
Gene symbol
MAPK8
Synonyms
c Jun N terminal kinase 1, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1

Background

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. MAPK8 functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. It has 4 isoforms produced by alternative splicing with the molecular weight of 46 kDa and 48 kDa. This protein can be phosphorylated and this antibody recognizes the 46 kDa and 55 kDa bands in western blot(PMID:11062067 ).


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