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JNK Antibody 66 Publications

Rabbit Polyclonal| Catalog number: 51151-1-AP

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Con: 28 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

JNK Antibody WB SH-SY5Y cells 51151-1-AP

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 51151-1-AP(JNK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

JNK Antibody IP HEK-293 cells 51151-1-AP

IP Result of anti-JNK (IP:51151-1-AP, 4ug; Detection:51151-1-AP 1:600) with HEK-293 cells lysate 2000ug.

JNK Antibody IF SH-SY5Y cells 51151-1-AP

Immunofluorescent analysis of ( -20℃ Acetone ) fixed SH-SY5Y cells using 51151-1-AP(JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, A. sphaerocephala Krasch, rat
Positive WB detected in:
SH-SY5Y cells, A431 cells, HEK-293 cells, HeLa cells, HT-1080 cells, PC-3 cells
Positive IP detected in:
HEK-293 cells
Positive IF detected in:
SH-SY5Y cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
mitogen-activated protein kinase 8
Calculated molecular weight:
48 kDa
Observed molecular weight:
44-48 kDa
GenBank accession number:
NM_138982
Gene ID (NCBI):
5599
Gene symbol
MAPK8
Synonyms
c Jun N terminal kinase 1, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1

Background

Function

JNK1, also known as mitogen-activated protein kinase 8 – MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

  • JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.  


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