Filter: AllWBIHCIF/ICC

WB analysis using 51151-1-AP
4T1 cells were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 51151-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 51151-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 51151-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 51151-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 51151-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 51151-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of SH-SY5Y using 51151-1-AP
Immunofluorescent analysis of (-20℃ Acetone) fixed SH-SY5Y cells using 51151-1-AP (JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

JNK Polyclonal antibody

Name: JNK Polyclonal antibody
Brand: Proteintech
SKU: 51151-1-AP

JNK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 51151-1-AP

Publications(128)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

c Jun N terminal kinase 1, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (128)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	4T1 cells, mouse brain tissue, A431 cells, HEK-293 cells, HepG2 cells, Jurkat cells, Neuro-2a cells, C6 cells, NIH/3T3 cells
Positive IHC detected in	human colon cancer tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 122 publications below
IHC	See 2 publications below
IP	See 2 publications below
CoIP	See 1 publications below

Product Information

51151-1-AP targets JNK in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	mitogen-activated protein kinase 8
Calculated Molecular Weight	48 kDa
Observed Molecular Weight	44-48 kDa, 50-55 kDa
GenBank Accession Number	NM_138982
Gene Symbol	JNK
Gene ID (NCBI)	5599
RRID	AB_2140710
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P45983
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.

Protocols

Product Specific Protocols
WB protocol for JNK antibody 51151-1-AP	Download protocol
IHC protocol for JNK antibody 51151-1-AP	Download protocol
IF protocol for JNK antibody 51151-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Res Mlkl knockout mice demonstrate the indispensable role of Mlkl in necroptosis. Authors - Wu Jianfeng J View Article
mouse	WB	J Crohns Colitis Mincle/Syk signaling promotes intestinal mucosal inflammation through induction of macrophage pyroptosis in Crohn's disease. Authors - Wenbin Gong View Article
Mouse	WB	Cell Death Differ TMCO1 is essential for ovarian follicle development by regulating ER Ca2+store of granulosa cells. Authors - Zhongshuai Sun View Article
mouse	WB	Br J Pharmacol Amentoflavone attenuates Listeria monocytogenes pathogenicity through an LLO-dependent mechanism. Authors - Wang Tingting View Article
mouse	WB	J Transl Med Angiotensin-(1-7) ameliorates sepsis-induced cardiomyopathy by alleviating inflammatory response and mitochondrial damage through the NF-κB and MAPK pathways Authors - Xin-Sen Chen View Article
human	WB	Biochim Biophys Acta Mol Basis Dis Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages Authors - Ling Zhou View Article