- Featured Product
- KD/KO Validated
JTV1 Polyclonal antibody
JTV1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10424-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, mouse liver tissue, K-562 cells, rat liver tissue, HepG2 cells, NIH/3T3 cells, L02 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human testis tissue, human cerebellum tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Hela cells, HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 14 publications below |
IF | See 3 publications below |
Product Information
10424-1-AP targets JTV1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | JTV1 fusion protein Ag0675 |
Full Name | JTV1 gene |
Calculated molecular weight | 36 kDa |
Observed molecular weight | 36 kDa |
GenBank accession number | BC002853 |
Gene symbol | JTV1 |
Gene ID (NCBI) | 7965 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
JTV1, also named p38, AIMP2, was first knew as a factor associated with macromolecular protein complex, which consists several different aminoacyl-tRNA synthetases. JTV1 is a scaffold protein required for the assembly and stability of the multi-tRNA synthetase complex. JTV1 could act as a mediator of TGF-beta signaling and its functional importance in the control of MYC during lung differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor. This is a rabbit polyclonal antibody raised against full-length of JTV1.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for JTV1 antibody 10424-1-AP | Download protocol |
IHC protocol for JTV1 antibody 10424-1-AP | Download protocol |
IF protocol for JTV1 antibody 10424-1-AP | Download protocol |
IP protocol for JTV1 antibody 10424-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Biol Chem Retractile lysyl-tRNA synthetase-AIMP2 assembly in the human multi-aminoacyl-tRNA synthetase complex. | ||
Materials (Basel) Fluorescence-Based Analysis of Noncanonical Functions of Aminoacyl-tRNA Synthetase-Interacting Multifunctional Proteins (AIMPs) in Peripheral Nerves. | ||
Int J Oncol Prostaglandin E2 promotes liver cancer cell growth by the upregulation of FUSE-binding protein 1 expression. | ||
Neuroscience Dysregulation of parkin in the substantia nigra of db/db and high-fat diet mice. | ||
Int J Mol Sci CRISPR-Cas9 Mediated Telomere Removal Leads to Mitochondrial Stress and Protein Aggregation. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH KRishnendu (Verified Customer) (02-27-2020) | Very strong antibody with no contaminant bands.
|