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JTV1 Antibody

JTV1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10424-1-AP

Synonyms

AIMP2, JTV1, JTV1 gene, P38, PRO0992, Protein JTV 1



Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, K-562 cells, rat liver tissue, HepG2 cells, NIH/3T3 cells, L02 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human cerebellum tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10424-1-AP targets JTV1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JTV1 fusion protein Ag0675
Full Name JTV1 gene
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession number BC002853
Gene symbol JTV1
Gene ID (NCBI) 7965
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

JTV1, also named p38, AIMP2, was first knew as a factor associated with macromolecular protein complex, which consists several different aminoacyl-tRNA synthetases. JTV1 is a scaffold protein required for the assembly and stability of the multi-tRNA synthetase complex. JTV1 could act as a mediator of TGF-beta signaling and its functional importance in the control of MYC during lung differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor. This is a rabbit polyclonal antibody raised against full-length of JTV1.

Protocols

Product Specific Protocols
WB protocol for JTV1 antibody 10424-1-APDownload protocol
IHC protocol for JTV1 antibody 10424-1-APDownload protocol
IF protocol for JTV1 antibody 10424-1-APDownload protocol
IP protocol for JTV1 antibody 10424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Retractile lysyl-tRNA synthetase-AIMP2 assembly in the human multi-aminoacyl-tRNA synthetase complex.

Authors - Zhoufei Hei
mouseWB

Materials (Basel)

Fluorescence-Based Analysis of Noncanonical Functions of Aminoacyl-tRNA Synthetase-Interacting Multifunctional Proteins (AIMPs) in Peripheral Nerves.

Authors - Muwoong Kim
mouseWB

Arch Neurol

Critical role of PINK1 in regulating Parkin protein levels in vivo.

Authors - Choo Yeun Su YS
humanWB

Int J Oncol

Prostaglandin E2 promotes liver cancer cell growth by the upregulation of FUSE-binding protein 1 expression.

Authors - Ma Juan J
mouseWB

Neuroscience

Dysregulation of parkin in the substantia nigra of db/db and high-fat diet mice.

Authors - Rin Khang
humanWB

Int J Mol Sci

CRISPR-Cas9 Mediated Telomere Removal Leads to Mitochondrial Stress and Protein Aggregation.

Authors - Hyojung Kim

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

KRishnendu (Verified Customer) (02-27-2020)

Very strong antibody with no contaminant bands.

  • Applications: Western Blot,Immunoprecipitation,
  • Primary Antibody Dilution: 1:2000 for WB; 1:100 for IP
  • Cell Tissue Type: HEK293T
No image submitted with review