Ajuba belongs to the LIM domain proteins, which are involved in the assembly of the extracellular matrix and, along with associated proteins, regulate target genes that connect the extracellular matrix and the cytoskeleton. Ajuba consists of three LIM domains at the C-terminal and is located in the cytoplasm where it interacts with a variety of other proteins. Ajuba contributes to the formation or strengthening of cell–cell adhesion and regulates cell growth and differentiation decisions. Ajuba participates in the assembly of the extracellular matrix. Ajuba also plays an important role in some diseases.