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LAMC3-Specific Monoclonal antibody

LAMC3-Specific Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4B8C5

Cat no : 67261-1-Ig

Synonyms

DKFZp434E202, LAMC3, LAMC3-Specific, Laminin 12 subunit gamma, Laminin 14 subunit gamma, Laminin 15 subunit gamma, Laminin subunit gamma 3, laminin, gamma 3

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using LAMC3-Specific Monoclonal antibody (67261-1-Ig)

    WB analysis of human testis using 67261-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 67261-1-Ig (LAMC3-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse placenta tissue using LAMC3-Specific Monoclonal antibody (67261-1-Ig)

    WB analysis of mouse placenta using 67261-1-Ig

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 67261-1-Ig (LAMC3-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using LAMC3-Specific Monoclonal antibody (67261-1-Ig)

    WB analysis of PC-3 using 67261-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 67261-1-Ig (LAMC3-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using LAMC3-Specific Monoclonal antibody (67261-1-Ig)

    IF Staining of mouse testis using 67261-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using LAMC3-Specific antibody (67261-1-Ig, Clone: 4B8C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman testis tissue, mouse placenta tissue, PC-3 cells
Positive IF detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67261-1-Ig targets LAMC3-Specific in WB, IF, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen LAMC3-Specific fusion protein Ag19568
Full Name laminin, gamma 3
Calculated Molecular Weight 1575 aa, 171 kDa
Observed Molecular Weight 180-200 kDa
GenBank Accession NumberNM_006059
Gene Symbol LAMC3
Gene ID (NCBI) 10319
RRIDAB_2882532
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LAMC3-Specific antibody 67261-1-IgDownload protocol
IF protocol for LAMC3-Specific antibody 67261-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols