LAMP1 Antibody

LAMP1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 55273-1-AP

Synonyms

CD107a, LAMP 1, LAMP1, LAMPA, LGP120



Tested Applications

Positive WB detected inHepG2 cells, human brain tissue
Positive IF detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55273-1-AP targets LAMP1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity Chinese soft-shelled turtles, human, turtle
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name lysosomal-associated membrane protein 1
Calculated molecular weight 45 kDa
Observed molecular weight 110 kDa
GenBank accession number NM_005561
Gene symbol LAMP1
Gene ID (NCBI) 3916
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.

Protocols

Product Specific Protocols
WB protocol for LAMP1 antibody 55273-1-APDownload protocol
IF protocol for LAMP1 antibody 55273-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
turtleIF

Reprod Biol Endocrinol

LIPOPHAGY: a novel form of steroidogenic activity within the LEYDIG cell during the reproductive cycle of turtle.

Authors - Imran Tarique
Chinese soft-shelled turtlesIF

Biomolecules

In Vivo Autophagy Up-Regulation of Small Intestine Enterocytes in Chinese Soft-Shelled Turtles during Hibernation.

Authors - Waseem Ali Vistro
human

J Neurochem

N-glycosylation of the AMPA-type glutamate receptor regulates cell surface expression and tetramer formation affecting channel function.

Authors - Munal Babu Kandel
turtleIF

Aging (Albany NY)

in vivo cellular evidence of autophagic associated spermiophagy within the principal cells during sperm storage in epididymis of the turtle.

Authors - Imran Tarique
humanWB, IF

Cell Death Dis

The lncRNA HULC functions as an oncogene by targeting ATG7 and ITGB1 in epithelial ovarian carcinoma.

Authors - Shuo Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Chun (Verified Customer) (12-05-2019)

a very good antibody

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: HeLa cells
No image submitted with review
FH

Cheryl (Verified Customer) (07-01-2019)

Robust antibody that works well for both IF and for Western blotting. Detects typical endosomal type structures by IF with little background and also picks up the multiple glycosylated forms of LAMP1 by Western blotting. Great value for money compared to alternatives from other suppliers.

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: A549 (human lung epithelial)
LAMP1 Antibody Western Blot,Immunofluorescence, validation (1:1000 dilution) in A549 (human lung epithelial) (Cat no:55273-1-AP)