LARG Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 22441-1-AP

  • Print page
  • Download PDF

-_-

Freight/Packing

Con: 74 μg/150 μl

Choose size:

Please visit your regions distributor:


Species specificity:
human

Positive WB detected in:
Jurkat cells, HeLa cells

Positive IHC detected in:
human testis tissue

Positive IF detected in:
HepG2 cells, HeLa cells

Recommended dilution:
WB : 1:1000-1:4000
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
Rho guanine nucleotide exchange factor (GEF) 12

Calculated molecular weight:
1544aa,173 kDa

Observed molecular weight:
220 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
ARHGEF12

Synonyms
ARHGEF12, DKFZp686O2372, KIAA0382, LARG, Leukemia associated RhoGEF, PRO2792
Background

LARG, also known as ARHGEF12, is one of three regulator of G protein signalling (RGS) domain-containing RhoGEFs. LARG activates the ROCK pathway downstream of Gα12/13 signaling, leading to cytoskeletal reorganisation.LARG performs this activity either as a homodimer or as a heterodimer with Arhgef11. LARG has been implicated in many diseases, including cancer, heart disease, and in HIV viral replication. LARG is phosphorylated and dephosphorylated during mitosis and that Cdk1 is the mitotic kinase responsible for LARG phosphorylation. We got 220 kDa in western blotting, maybe due to its phosphorylation.


Back
to top