|Positive WB detected in||mouse liver tissue, A431 cells, A549 cells, HeLa cells, Jurkat cells, L02 cells, rat liver tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||L02 cells, HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11836-1-AP targets LBP in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||LBP fusion protein Ag2431|
|Full Name||lipopolysaccharide binding protein|
|Calculated molecular weight||481 aa, 53 kDa|
|Observed molecular weight||63-67 kDa|
|GenBank accession number||BC022256|
|Gene ID (NCBI)||3929|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
LBP belongs to the BPI/LBP/Plunc superfamily and BPI/LBP family. It binds to the lipid A moiety of bacterial lipopolysaccharides (LPS) which is a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In many tissue/cells, we get 53-60kd and 63-67kd(Glycosylation form).
|Product Specific Protocols|
|WB protocol for LBP antibody 11836-1-AP||Download protocol|
|IHC protocol for LBP antibody 11836-1-AP||Download protocol|
|IF protocol for LBP antibody 11836-1-AP||Download protocol|
|IP protocol for LBP antibody 11836-1-AP||Download protocol|
|FC protocol for LBP antibody 11836-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Biomed Mater Res A
The characteristic regulation of gene expression Lbp and Sod3 in peri-implant connective tissue of rats.
Circulating exosomes contain protein biomarkers of metastatic non-small-cell lung cancer.
Association of decreased levels of lipopolysaccharide-binding protein with OKN-007-induced regression of tumor growth in an F98 rat glioma model.