LBP Polyclonal antibody

LBP Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11836-1-AP

Synonyms

LBP



Tested Applications

Positive WB detected inmouse liver tissue, A431 cells, A549 cells, HeLa cells, Jurkat cells, L02 cells, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inL02 cells, HepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11836-1-AP targets LBP in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LBP fusion protein Ag2431
Full Name lipopolysaccharide binding protein
Calculated molecular weight 481 aa, 53 kDa
Observed molecular weight 63-67 kDa
GenBank accession numberBC022256
Gene symbol LBP
Gene ID (NCBI) 3929
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LBP belongs to the BPI/LBP/Plunc superfamily and BPI/LBP family. It binds to the lipid A moiety of bacterial lipopolysaccharides (LPS) which is a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In many tissue/cells, we get 53-60kd and 63-67kd(Glycosylation form).

Protocols

Product Specific Protocols
WB protocol for LBP antibody 11836-1-APDownload protocol
IHC protocol for LBP antibody 11836-1-APDownload protocol
IF protocol for LBP antibody 11836-1-APDownload protocol
IP protocol for LBP antibody 11836-1-APDownload protocol
FC protocol for LBP antibody 11836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

J Biomed Mater Res A

The characteristic regulation of gene expression Lbp and Sod3 in peri-implant connective tissue of rats.

Authors - Takafumi Kobayashi
humanWB

Cancer Sci

Circulating exosomes contain protein biomarkers of metastatic non-small-cell lung cancer.

Authors - Ning Wang

J Neurosurg

Association of decreased levels of lipopolysaccharide-binding protein with OKN-007-induced regression of tumor growth in an F98 rat glioma model.

Authors - Nataliya Smith