LBR Polyclonal antibody
LBR Polyclonal Antibody for FC, IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 12398-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A375 cells, human brain tissue, C2C12 cells |
| Positive IP detected in | A375 cells |
| Positive IF detected in | Hela cells, C6 cells, HepG2 cells |
| Positive FC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunofluorescence (IF) | IF : 1:25-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 3 publications below |
| IF | See 3 publications below |
Product Information
12398-1-AP targets LBR in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LBR fusion protein Ag3088 |
| Full Name | lamin B receptor |
| Calculated molecular weight | 615 aa, 71 kDa |
| Observed molecular weight | 58 kDa |
| GenBank accession number | BC020079 |
| Gene symbol | LBR |
| Gene ID (NCBI) | 3930 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Lamin-B receptor (LBR) belongs to the ERG4/ERG24 family. LBR is a 58-kDa integral protein of the nuclear envelope inner membrane and anchors the lamina and the heterochromatin to the inner nuclear membrane. LBR may mediate interaction between chromatin and lamin B. Mutations of the LBR gene has been associated with autosomal recessive HEM/Greenberg skeletal dysplasia.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LBR antibody 12398-1-AP | Download protocol |
| IF protocol for LBR antibody 12398-1-AP | Download protocol |
| IP protocol for LBR antibody 12398-1-AP | Download protocol |
| FC protocol for LBR antibody 12398-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS Genet RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases. | ||
Mol Cell Biol Interactome analysis reveals a novel role for RAD6 in the regulation of proteasome activity and localization in response to DNA damage. | ||
J Cell Sci Emery-Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane. | ||
Nat Commun PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments. |
