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LBR Polyclonal antibody

LBR Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 12398-1-AP

Synonyms

DHCR14B, lamin B receptor, LBR, LMN2R, PHA

Validation Data Gallery

  • WB analysis of A375 using 12398-1-AP

    WB analysis of A375 using 12398-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12398-1-AP (LBR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of Hela using 12398-1-AP

    IF Staining of Hela using 12398-1-AP

    Immunofluorescent analysis of Hela cells, using LBR antibody 12398-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of A375 using 12398-1-AP

    IP experiment of A375 using 12398-1-AP

    IP Result of anti-LBR (IP:12398-1-AP, 3ug; Detection:12398-1-AP 1:500) with A375 cells lysate 3200ug.

  • FC experiment of Jurkat using 12398-1-AP

    FC experiment of Jurkat using 12398-1-AP

    1X10^6 Jurkat cells were stained with 0.2ug LBR antibody (12398-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inA375 cells, human brain tissue, C2C12 cells
Positive IP detected inA375 cells
Positive IF detected inHela cells, C6 cells, HepG2 cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:25-1:100
Sample-dependent, check data in validation data gallery

Product Information

12398-1-AP targets LBR in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LBR fusion protein Ag3088
Full Name lamin B receptor
Calculated molecular weight 615 aa, 71 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC020079
Gene symbol LBR
Gene ID (NCBI) 3930
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lamin-B receptor (LBR) belongs to the ERG4/ERG24 family. LBR is a 58-kDa integral protein of the nuclear envelope inner membrane and anchors the lamina and the heterochromatin to the inner nuclear membrane. LBR may mediate interaction between chromatin and lamin B. Mutations of the LBR gene has been associated with autosomal recessive HEM/Greenberg skeletal dysplasia.

Protocols

Product Specific Protocols
WB protocol for LBR antibody 12398-1-APDownload protocol
IF protocol for LBR antibody 12398-1-APDownload protocol
IP protocol for LBR antibody 12398-1-APDownload protocol
FC protocol for LBR antibody 12398-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

JCI Insight

A common pathomechanism in GMAP-210- and LBR-related diseases.

Authors - Anika Wehrle
View Article
humanWB

PLoS Genet

RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases.

Authors - Aurelio Reyes
View Article
humanWB,IF

Mol Cell Biol

Interactome analysis reveals a novel role for RAD6 in the regulation of proteasome activity and localization in response to DNA damage.

Authors - Hongli An
View Article
humanIF

J Cell Sci

Emery-Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane.

Authors - Janine Pfaff
View Article

Nat Commun

PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments.

Authors - Chen-Yu Wang
View Article