Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of HAP1 using 12398-1-AP
WB result of LBR antibody (12398-1-AP; 1:40000; room temperature for 1.5 hours) with wild-type and LBR knockout HAP1 cells.
WB analysis using 12398-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12398-1-AP (LBR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 12398-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 12398-1-AP (LBR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12398-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12398-1-AP (LBR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of C2C12 using 12398-1-AP
C2C12 cells were subjected to SDS PAGE followed by western blot with 12398-1-AP (LBR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of A375 using 12398-1-AP
IP result of anti-LBR (IP:12398-1-AP, 3ug; Detection:12398-1-AP 1:500) with A375 cells lysate 3200ug.
IHC staining of human skin cancer using 12398-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 12398-1-AP (LBR antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 12398-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 12398-1-AP (LBR antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 12398-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 12398-1-AP (LBR antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 12398-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LBR antibody (12398-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HeLa using 12398-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LBR antibody (12398-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of HepG2 using 12398-1-AP
Immunofluorescent analysis of HepG2 cells, using LBR antibody 12398-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HeLa using 12398-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12398-1-AP (LBR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of C6 using 12398-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using 12398-1-AP (LBR antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 12398-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human LBR (12398-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) at dilution 1:1000(red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Featured Product
KD/KO Validated

LBR Polyclonal antibody

LBR Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 12398-1-AP

Publications(11)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Synonyms

14, 3-beta-hydroxysterol Delta, C-14 sterol reductase, C14SR, Delta(14)-sterol reductase LBR

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®555

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information
Publications (11)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, A375 cells, C2C12 cells, HAP1 cells, human brain tissue, HeLa cells, HepG2 cells
Positive IP detected in	A375 cells
Positive IHC detected in	human skin cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells, C6 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below
IF	See 4 publications below
IP	See 1 publications below
CoIP	See 1 publications below
ChIP	See 1 publications below

Product Information

12398-1-AP targets LBR in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LBR fusion protein Ag3088 Predict reactive species
Full Name	lamin B receptor
Calculated Molecular Weight	615 aa, 71 kDa
Observed Molecular Weight	58 kDa
GenBank Accession Number	BC020079
Gene Symbol	LBR
Gene ID (NCBI)	3930
RRID	AB_2138334
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14739
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lamin-B receptor (LBR) is an integral membrane protein of the inner nuclear membrane that contains a hydrophilic N-terminal end protruding into the nucleoplasm, eight hydrophobic segments that span the membrane and a short, nucleoplasmic C-terminal tail (PMID: 28858257). LBR anchors the lamina and the heterochromatin to the inner nuclear membrane (PMID: 10828963; 28858257). It is also essential for cholesterol synthesis (PMID: 27336722). Mutations of the LBR gene have been associated with autosomal recessive HEM/Greenberg skeletal dysplasia and Pelger-Huët anomaly and Greenberg skeletal dysplasia (PMID: 12618959; 12490533). The calculated molecular mass of LBR is 71 kDa, which is larger than the apparent molecular mass of 58 kDa, probably due to the aberrant migration of membrane proteins subjected to SDS-PAGE (PMID: 2847165; 2170422).

Protocols

Product Specific Protocols
WB protocol for LBR antibody 12398-1-AP	Download protocol
IHC protocol for LBR antibody 12398-1-AP	Download protocol
IF protocol for LBR antibody 12398-1-AP	Download protocol
IP protocol for LBR antibody 12398-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Mol Cell Interaction of tau with HNRNPA2B1 and N6-methyladenosine RNA mediates the progression of tauopathy. Authors - Lulu Jiang View Article
		Nat Commun PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments. Authors - Chen-Yu Wang View Article
human	WB	PLoS Genet RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases. Authors - Aurelio Reyes View Article
human	IF	J Cell Sci Emery-Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane. Authors - Janine Pfaff View Article
human	WB,IP	J Cell Mol Med Novel long non-coding RNA CYB561-5 promotes aerobic glycolysis and tumorigenesis by interacting with basigin in non-small cell lung cancer. Authors - Longfei Li View Article
human	WB,IF	Mol Cell Biol Interactome analysis reveals a novel role for RAD6 in the regulation of proteasome activity and localization in response to DNA damage. Authors - Hongli An View Article