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LDHA-Specific Antibody 49 Publications

Rabbit Polyclonal| Catalog number: 19987-1-AP

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Con: 39 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

LDHA-Specific Antibody WB HEK-293 cells 19987-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

LDHA-Specific Antibody IF HepG2 cells 19987-1-AP

Immunofluorescent analysis of HepG2 cells using 19987-1-AP(LDHA-Specific antibody) at dilution of 1:100 and Rhodamine-Goat anti-Rabbit IgG

LDHA-Specific Antibody IP mouse kidney tissue 19987-1-AP

IP Result of anti-LDHA-Specific (IP:19987-1-AP, 4ug; Detection:19987-1-AP 1:2000) with mouse kidney tissue lysate 4000ug.

LDHA-Specific Antibody IHC human heart tissue 19987-1-AP

Immunohistochemical analysis of paraffin-embedded human heart using 19987-1-AP(LDHA-Specific antibody) at dilution of 1:100 (under 10x lens)

LDHA-Specific Antibody IHC human heart tissue 19987-1-AP

Immunohistochemical analysis of paraffin-embedded human heart using 19987-1-AP(LDHA-Specific antibody) at dilution of 1:100 (under 40x lens)

LDHA-Specific Antibody FC HepG2 cells 19987-1-AP

1X10^6 HepG2 cells were stained with 0.2ug LDHA-Specific antibody (19987-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
HEK-293 cells, Jurkat cells, A431 cells, mouse testis tissue, rat kidney tissue, HeLa cells, NIH/3T3 cells
Positive IP detected in:
mouse kidney tissue
Positive IHC detected in:
human heart tissue, human skeletal muscle tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells, Hela cells
Positive FC detected in:
HepG2 cells
Recommended dilution:
WB : 1:2000-1:10000
IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
lactate dehydrogenase A
Calculated molecular weight:
37 kDa
Observed molecular weight:
32-37 kDa
GenBank accession number:
NM_005566
Gene ID (NCBI):
3939
Gene symbol
LDHA
Synonyms
lactate dehydrogenase A, LDH A, LDH M, LDH muscle subunit, LDH1, LDHA, PIG19

Background

 Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087). This antibody is specific to LDHA and has no cross reaction to LDHB and LDHC.


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