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- KD/KO Validated
LDHA-Specific Polyclonal antibody
LDHA-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat no : 19987-1-AP
|Positive WB detected in||HEK-293 cells, Jurkat cells, A431 cells, mouse testis tissue, rat kidney tissue, HeLa cells, NIH/3T3 cells|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human heart tissue, human skeletal muscle tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, Hela cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
19987-1-AP targets LDHA-Specific in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||lactate dehydrogenase A|
|Calculated molecular weight||37 kDa|
|Observed molecular weight||32-37 kDa|
|GenBank accession number||NM_005566|
|Gene ID (NCBI)||3939|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087). This antibody is specific to LDHA and has no cross reaction to LDHB and LDHC.
|Product Specific Protocols|
|WB protocol for LDHA-Specific antibody 19987-1-AP||Download protocol|
|IHC protocol for LDHA-Specific antibody 19987-1-AP||Download protocol|
|IF protocol for LDHA-Specific antibody 19987-1-AP||Download protocol|
|IP protocol for LDHA-Specific antibody 19987-1-AP||Download protocol|
|FC protocol for LDHA-Specific antibody 19987-1-AP||Download protocol|
|Click here to view our Standard Protocols|
p53/Lactate dehydrogenase A axis negatively regulates aerobic glycolysis and tumor progression in breast cancer expressing wild-type p53.
The SIAH2-NRF1 axis spatially regulates tumor microenvironment remodeling for tumor progression.
Cell Commun Signal
Blockade of glycolysis-dependent contraction by oroxylin a via inhibition of lactate dehydrogenase-a in hepatic stellate cells.
Int J Biol Sci
The KLF14 Transcription Factor Regulates Glycolysis by Downregulating LDHB in Colorectal Cancer.
UHRF1 promotes aerobic glycolysis and proliferation via suppression of SIRT4 in pancreatic cancer.
Homeodomain-interacting protein kinase 2 suppresses proliferation and aerobic glycolysis via ERK/cMyc axis in pancreatic cancer.