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KD/KO Validated

LDHA-Specific Polyclonal antibody

Name: LDHA-Specific Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 19987-1-AP

LDHA-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 19987-1-AP

Synonyms

lactate dehydrogenase A, LDH A, LDH M, LDH muscle subunit, LDH1, LDHA, PIG19

Validation Data Gallery

WB analysis using 19987-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IF Staining of HepG2 using 19987-1-AP
Immunofluorescent analysis of HepG2 cells using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 and Rhodamine-Goat anti-Rabbit IgG.
IP experiment of mouse kidney using 19987-1-AP
IP Result of anti-LDHA-Specific (IP:19987-1-AP, 4ug; Detection:19987-1-AP 1:2000) with mouse kidney tissue lysate 4000ug.
IHC staining of human heart using 19987-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 19987-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 40x lens).
FC experiment of HepG2 using 19987-1-AP
1X10^6 HepG2 cells were stained with 0.2ug LDHA-Specific antibody (19987-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Cat No. 19987-1-AP

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Tested Applications

Positive WB detected in	HEK-293 cells, Jurkat cells, A431 cells, mouse testis tissue, rat kidney tissue, HeLa cells, NIH/3T3 cells
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human heart tissue, human skeletal muscle tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells, Hela cells
Positive FC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)	IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 4 publications below
WB	See 57 publications below
IHC	See 9 publications below
IF	See 1 publications below
IP	See 2 publications below

Product Information

19987-1-AP targets LDHA-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide
Full Name	lactate dehydrogenase A
Calculated molecular weight	37 kDa
Observed molecular weight	32-37 kDa
GenBank accession number	NM_005566
Gene symbol	LDHA
Gene ID (NCBI)	3939
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087). This antibody is specific to LDHA and has no cross reaction to LDHB and LDHC.

Protocols

Product Specific Protocols
WB protocol for LDHA-Specific antibody 19987-1-AP	Download protocol
IHC protocol for LDHA-Specific antibody 19987-1-AP	Download protocol
IF protocol for LDHA-Specific antibody 19987-1-AP	Download protocol
IP protocol for LDHA-Specific antibody 19987-1-AP	Download protocol
FC protocol for LDHA-Specific antibody 19987-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Cancer Sci p53/Lactate dehydrogenase A axis negatively regulates aerobic glycolysis and tumor progression in breast cancer expressing wild-type p53. Authors - Yao Zhou View Article
human	WB	Nat Commun The SIAH2-NRF1 axis spatially regulates tumor microenvironment remodeling for tumor progression. Authors - Biao Ma View Article
human	WB,IF	Cell Commun Signal Blockade of glycolysis-dependent contraction by oroxylin a via inhibition of lactate dehydrogenase-a in hepatic stellate cells. Authors - Feixia Wang KD Validated View Article
human	WB,IHC	Int J Biol Sci The KLF14 Transcription Factor Regulates Glycolysis by Downregulating LDHB in Colorectal Cancer. Authors - Guiyang Wu View Article
human	WB	Cancer Lett UHRF1 promotes aerobic glycolysis and proliferation via suppression of SIRT4 in pancreatic cancer. Authors - Qiangsheng Hu View Article
human	WB	Cell Prolif Homeodomain-interacting protein kinase 2 suppresses proliferation and aerobic glycolysis via ERK/cMyc axis in pancreatic cancer. Authors - Yi Qin View Article