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LDHB Polyclonal antibody

LDHB Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14824-1-AP

Synonyms

lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, TRG 5



Tested Applications

Positive WB detected inDU 145 cells, HeLa cells, mouse brain tissue, PC-3 cells, rat brain tissue, rat liver tissue, mouse heart tissue, rat heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:400-1:1500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14824-1-AP targets LDHB in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LDHB fusion protein Ag6605
Full Name lactate dehydrogenase B
Calculated molecular weight 36 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC002362
Gene symbol LDHB
Gene ID (NCBI) 3945
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of pyruvate and NADH to lactate and NAD+ in the glycolytic pathway (PMID: 26269128). It is glycolytic enzymes and it is positively regulated by mTOR.

Protocols

Product Specific Protocols
WB protocol for LDHB antibody 14824-1-APDownload protocol
IHC protocol for LDHB antibody 14824-1-APDownload protocol
IF protocol for LDHB antibody 14824-1-APDownload protocol
IP protocol for LDHB antibody 14824-1-APDownload protocol
FC protocol for LDHB antibody 14824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Res

Retinoic acid-related orphan receptor C regulates proliferation, glycolysis, and chemoresistance via the PD-L1/ITGB6/STAT3 signaling axis in bladder cancer.

Authors - Dalong Cao

Mol Med Rep

Fructose‑1,6‑bisphosphatase‑1 decrease may promote carcinogenesis and chemoresistance in cervical cancer.

Authors - Haoran Li
humanWB,IHC

Am J Cancer Res

PMID: 27294003

Authors - Shaojia Wang
mouseIF

Invest Ophthalmol Vis Sci

Laser capture microdissection-directed profiling of glycolytic and mTOR pathways in areas of selectively ablated Müller cells in the murine retina.

Authors - Chung Sook Hyun SH
humanWB

Am J Cancer Res

Bufalin inhibits glycolysis-induced cell growth and proliferation through the suppression of Integrin β2/FAK signaling pathway in ovarian cancer.

Authors - Haoran Li
humanWB

PLoS One

Probing early tumor response to radiation therapy using hyperpolarized [1-¹³C]pyruvate in MDA-MB-231 xenografts.

Authors - Chen Albert P AP