|Positive WB detected in||DU 145 cells, HeLa cells, mouse brain tissue, PC-3 cells, rat brain tissue, rat liver tissue, mouse heart tissue, rat heart tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human kidney tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:400-1:1500|
|Sample-dependent, check data in validation data gallery|
14824-1-AP targets LDHB in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||LDHB fusion protein Ag6605|
|Full Name||lactate dehydrogenase B|
|Calculated molecular weight||36 kDa|
|Observed molecular weight||35 kDa|
|GenBank accession number||BC002362|
|Gene ID (NCBI)||3945|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of pyruvate and NADH to lactate and NAD+ in the glycolytic pathway (PMID: 26269128). It is glycolytic enzymes and it is positively regulated by mTOR.
|Product Specific Protocols|
|WB protocol for LDHB antibody 14824-1-AP||Download protocol|
|IHC protocol for LDHB antibody 14824-1-AP||Download protocol|
|IP protocol for LDHB antibody 14824-1-AP||Download protocol|
|FC protocol for LDHB antibody 14824-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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