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LDLR Polyclonal antibody

LDLR Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (5)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10785-1-AP

Synonyms

FH, FHC, LDL receptor, LDLR



Tested Applications

Positive WB detected inHeLa cells, HL-60 cells, NIH/3T3 cells, mouse brain tissue, Jurkat cells, human brain tissue, Raji cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue, human colon cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10785-1-AP targets LDLR in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity Chicken, Hamster, human, Landrace, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LDLR fusion protein Ag1236
Full Name low density lipoprotein receptor
Calculated molecular weight 95 kDa
Observed molecular weight 150-160 kDa, 120 kDa
GenBank accession numberBC014514
Gene symbol LDLR
Gene ID (NCBI) 3949
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.

Protocols

Product Specific Protocols
WB protocol for LDLR antibody 10785-1-APDownload protocol
IHC protocol for LDLR antibody 10785-1-APDownload protocol
IF protocol for LDLR antibody 10785-1-APDownload protocol
IP protocol for LDLR antibody 10785-1-APDownload protocol
FC protocol for LDLR antibody 10785-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Redox Biol

Endogenous cholesterol ester hydroperoxides modulate cholesterol levels and inhibit cholesterol uptake in hepatocytes and macrophages.

Authors - Shuyuan Guo
ratWB

Molecules

Therapeutic Effect of Chitooligosaccharide Tablets on Lipids in High-Fat Diets Induced Hyperlipidemic Rats.

Authors - Di Yang
humanWB

Sci Rep

PMID: 30816192

Authors - Lin Zhu
human,mouseWB

Nat Commun

Liver-target nanotechnology facilitates berberine to ameliorate cardio-metabolic diseases.

Authors - Hui-Hui Guo

Int J Nanomedicine

A Protein Corona Adsorbed to a Bacterial Magnetosome Affects Its Cellular Uptake.

Authors - Wenjia Lai
humanWB,IF

Ann Biomed Eng

A Novel Role of Id1 in Regulating Oscillatory Shear Stress-Mediated Lipid Uptake in Endothelial Cells.

Authors - Kang Zhang
  • KD Validated