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  • KD/KO Validated

LDLR Polyclonal antibody

LDLR Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10785-1-AP

Synonyms

FH, FHC, LDL receptor, LDLR



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, human brain tissue, Raji cells, mouse brain tissue, HL-60 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue, human brain tissue, mouse liver tissue, human pancreas tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10785-1-AP targets LDLR in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, Chicken, Hamster, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LDLR fusion protein Ag1236
Full Name low density lipoprotein receptor
Calculated Molecular Weight 95 kDa
Observed Molecular Weight 100-160 kDa
GenBank Accession NumberBC014514
Gene Symbol LDLR
Gene ID (NCBI) 3949
RRIDAB_2281164
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.

Protocols

Product Specific Protocols
WB protocol for LDLR antibody 10785-1-APDownload protocol
IHC protocol for LDLR antibody 10785-1-APDownload protocol
IF protocol for LDLR antibody 10785-1-APDownload protocol
IP protocol for LDLR antibody 10785-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Authors - Yi Fu
humanWB

Cell Metab

Identification and evaluation of a lipid-lowering small compound in preclinical models and in a Phase I trial.

Authors - Jiang Wang
human,mouseWB,IF

Sci Transl Med

Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology

Authors - Su'an Tang
mouseWB,IP

J Clin Invest

Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.

Authors - Shanshan Zhong
  • KO Validated
humanWB

Nat Commun

A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.

Authors - Yunkai Zhu
mouseIF

Adv Healthc Mater

Engineered Red Blood Cell Membrane-Coating Salidroside/Indocyanine Green Nanovesicles for High-Efficiency Hypoxic Targeting Phototherapy of Triple-Negative Breast Cancer.

Authors - Yi Pan