Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, L02 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human brain tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10785-1-AP targets LDLR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig, chicken, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1236 Product name: Recombinant human LDLR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC014514 Sequence: MGPWGWKLRWTVALLLAAAGTAVGDRCERNEFQCQDGKCISYKWVCDGSAECQDGSDESQETCLSVTCKSGDFSCGGRVNRCIPQFWRCDGQVDCDNGSDEQGCPPKTCSQDEFRCHDGKCISRQFVCDSDRDCLDGSDEASCPVLTCGPASFQCNSSTCIPQLWACDNDPDCEDGSDEWPQRCRGLYVFQGDSSPCSAFEFHCLSGECIHSSWRCDGGPDCKDKSDEENCAVATCRPDEFQCSDGNCIHGSRQCDREYDCKDMSDEVGCVNVTLCEGPNKFKCHSGECITLDKVCNMARDCRDWSDEPIKECGTNECLDNNGGCSHVCNDLKIGYECLCPDGFQLVAQR Predict reactive species |
| Full Name | low density lipoprotein receptor |
| Calculated Molecular Weight | 95 kDa |
| Observed Molecular Weight | 100-160 kDa |
| GenBank Accession Number | BC014514 |
| Gene Symbol | LDLR |
| Gene ID (NCBI) | 3949 |
| ENSEMBL Gene ID | ENSG00000130164 |
| RRID | AB_2281164 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01130 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LDLR antibody 10785-1-AP | Download protocol |
| IHC protocol for LDLR antibody 10785-1-AP | Download protocol |
| IP protocol for LDLR antibody 10785-1-AP | Download protocol |
| WB protocol for LDLR antibody 10785-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LDLR Polyclonal antibody (Cat# 10785-1-AP) has accumulated 160 citations across high-impact journals including Cell, Nature Biotechnology, Nature Communications, Nature Nanotechnology, Molecular Cell, Science Translational Medicine, and the Journal of Clinical Investigation. Research fields span cholesterol and lipid metabolism, atherosclerosis, cardiovascular disease, cancer biology, neurodegeneration, viral entry mechanisms, drug discovery, and metabolic disorders such as diabetes and NAFLD. The antibody is predominantly applied in Western blot, immunofluorescence, immunohistochemistry, and immunoprecipitation.
| Species | Application | Title |
|---|---|---|
Cell A commonly inherited human PCSK9 germline variant drives breast cancer metastasis via LRP1 receptor | ||
Mol Cancer Systemic immunosuppression limits NK cell therapy efficacy in pancreatic cancer. | ||
Reviews
What customers say
The single review reports a 5-star rating for this antibody used in immunofluorescence on PFA-fixed mouse primary cells at a 1:250 dilution. The user found it performed well for their application. Overall, the limited feedback is positive, with the reviewer confirming successful use in IF on mouse cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Katia (Verified Customer) (09-11-2025) | Used for immunofluorescence on PFA-fixed mouse primary cells.
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