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  • KD/KO Validated

LGALS3BP Polyclonal antibody

LGALS3BP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10281-1-AP

Synonyms

90K, BTBD17B, Galectin 3 binding protein, LGALS3BP, M2BP, Mac 2 binding protein, MAC 2 BP, MAC2BP, Tumor associated antigen 90K



Tested Applications

Positive WB detected infetal human brain tissue, HEK-293 cells, A549 cells, HeLa cells, COLO 320 cells
Positive IP detected inHepG2 cells, HEK-293 cells
Positive IHC detected inhuman oesophagus cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10281-1-AP targets LGALS3BP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LGALS3BP fusion protein Ag0294
Full Name lectin, galactoside-binding, soluble, 3 binding protein
Calculated molecular weight 585 aa, 65 kDa
Observed molecular weight 65-90 kDa
GenBank accession numberBC002403
Gene symbol LGALS3BP
Gene ID (NCBI) 3959
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ).

Protocols

Product Specific Protocols
WB protocol for LGALS3BP antibody 10281-1-APDownload protocol
IHC protocol for LGALS3BP antibody 10281-1-APDownload protocol
IF protocol for LGALS3BP antibody 10281-1-APDownload protocol
IP protocol for LGALS3BP antibody 10281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Blood Adv

Increased Mac-2 binding protein glycan isomer in patients at risk for late nonrelapse mortality after HSCT.

Authors - Yu Akahoshi
mouseIF

Mol Med Rep

Composition and characteristics of distinct macrophage subpopulations in the mouse thymus.

Authors - Lan-Tao Liu
humanWB

Haemophilia

Galectin 3-binding protein is a potential contaminant of recombinantly produced factor IX.

Authors - Blostein M M
humanIHC

Cancer Biol Ther

SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome.

Authors - Kashyap Manoj Kumar MK
humanWB,IHC

Cell Signal

Increased LGALS3BP promotes proliferation and migration of oral squamous cell carcinoma via PI3K/AKT pathway.

Authors - Xiaoxin Zhang
  • KD Validated
humanWB

PLoS One

O-GlcNAc-specific antibody CTD110.6 cross-reacts with N-GlcNAc2-modified proteins induced under glucose deprivation.

Authors - Isono Takahiro T