Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18051-1-AP targets DNA Ligase I in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12508 Product name: Recombinant human LIG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 670-919 aa of BC108318 Sequence: LVREPLSRRRQLLRENFVETEGEFVFATSLDTKDIEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLGRGKRAGRYGGFLLASYDEDSEELQAICKLGTGFSDEELEEHHQSLKALVLPSPRPYVRIDGAVIPDHWLDPSAVWEVKCADLSLSPIYPAARGLVDSDKGISLRFPRFIRVREDKQPEQATTSAQVACLYRKQSQIQNQQGEDSGSDPEDTY Predict reactive species |
| Full Name | ligase I, DNA, ATP-dependent |
| Calculated Molecular Weight | 919 aa, 102 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC108318 |
| Gene Symbol | LIG1 |
| Gene ID (NCBI) | 3978 |
| RRID | AB_2265726 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P18858 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNA ligase I (LIG1) joins DNA strand breaks during DNA replication and repair transactions and contributes to genome integrity. 18051-1-AP is raised agains the C-terminal 670-919 aa residues of the human DNA ligase 1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DNA Ligase I antibody 18051-1-AP | Download protocol |
| IP protocol for DNA Ligase I antibody 18051-1-AP | Download protocol |
| WB protocol for DNA Ligase I antibody 18051-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-LIG1 (DNA Ligase I) Affinity Purified Rabbit mAb (Cat# 18051-1-AP) has 17 total citations. These appear in journals including Nature, Molecular Cell, Nature Communications, PNAS, Journal of Clinical Investigation, Science Advances, Cancer Discovery, Molecular Cancer, Nucleic Acids Research, Cell Reports, Scientific Reports, Journal of Molecular Biology, Epigenetics & Chromatin, and bioRxiv. Research fields span DNA damage repair, genome stability, cancer biology, immunodeficiency, V(D)J recombination, and epigenetics.
| Species | Application | Title |
|---|---|---|
Mol Cancer hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma | ||
Cancer Discov Proteogenomic Markers of Chemotherapy Resistance and Response in Triple-Negative Breast Cancer | ||
Nat Commun Cas9-induced large deletions and small indels are controlled in a convergent fashion. | ||
Nucleic Acids Res Lig3-dependent rescue of mouse viability and DNA double-strand break repair by catalytically inactive Lig4 |
