LIG3 Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 26583-1-AP

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Freight/Packing

Con: 75 μg/150 μl

Choose size:

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Species specificity:
human, mouse

Positive WB detected in:
HEK-293T cells

Positive IP detected in:
HEK-293T cells

Positive IF detected in:
HeLa cells

Recommended dilution:
WB : 1:1000-1:8000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
ligase III, DNA, ATP-dependent

Calculated molecular weight:

Observed molecular weight:
100 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
LIG3

Synonyms
DNA ligase 3, DNA ligase III, LIG2, LIG3, ligase III, DNA, ATP dependent
Background

DNA ligase 3 is an enzyme that in humans is encoded by the LIG3 gene. The human LIG3 gene encodes ATP-dependent DNA ligases that seal interruptions in the phosphodiester backbone of duplex DNA. There are three families of ATP-dependent DNA ligases in eukaryotes. These enzymes utilize the same three step reaction mechanism; 1 formation of a covalent enzyme-adenylate intermediate; 2 transfer of the adenylate group to the 5’ phosphate terminus of a DNA nick; 3 phosphodiester bond formation. Unlike LIG1 and LIG4 family members that are found in almost all eukaryotes, LIG3 family members are less widely distributed. The LIG3 gene encodes several distinct DNA ligase species by alternative translation initiation and alternative splicing mechanisms


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